RPS14 Antibody - BSA Free

Western Blot: RPS14 Antibody [NBP2-22319] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. NBP2-22319 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: RPS14 Antibody [NBP2-22319] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22319 used for IP at 6 ug/mg lysate. RPS14 was also immunoprecipitated by rabbit anti-RPS14 antibody. For blotting immunoprecipitated RPS14, NBP2-22319 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu, Rt, Dr
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RPS14 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RPS14 Antibody - BSA Free (NBP2-22319) is a polyclonal antibody validated for use in WB and IP. Anti-RPS14 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 101 to 151 of human Ribosomal Protein S14 using the numbering given in entry NP_001020242.1 (GeneID 6208).
• Predicted Species: Rat (100%), Drosophila (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-12% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RPS14 Antibody - BSA Free

• emetine resistance
• EMTB40S ribosomal protein S14
• ribosomal protein S14

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPS14 Antibody (NBP2-22319)(1)

Publication using NBP2-22319

• Lafita-Navarro MC, Hao YH, Jiang C et al. ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells Cell reports 2023-10-31 [PMID: 37851577] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RPS14
• Uniprot:
  • NP_001020242.1(Human)