RNF2 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RNF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RNF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

RNF2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human RNF2
  aa 147-228
  Accession # Q99496
• Specificity: Detects human, mouse, and rat RNF2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RNF2 Antibody [Unconjugated]

• BAP-1
• BAP1RING finger protein 1B
• DING
• DINGRING finger protein BAP-1
• EC 6.3.2
• HIPI3
• HIPI3RING1BE3 ubiquitin-protein ligase RING2
• Huntingtin-interacting protein 2-interacting protein 3
• Protein DinG
• ring finger protein 2HIP2-interacting protein 3
• RING1B
• RING2
• RING2EC 6.3.2.-
• RNF2

Background

RNF2 (RING [really interesting gene] finger protein 2/1B; also BAP-1, HIP-2 interacting protein 3 and DinG) is a 37 kDa member of the ring finger domain family of proteins. It is expressed in embryonic tissue, and serves as an E3 ubiquitin ligase within a polycomb complex that acts on histone H2A. In effect, RNF2 contributes to gene repression by blocking RNA pol II advance along gene promoters. RNF2 forms homodimers, and interacts with over a dozen proteins. Human RNF2 is 336 amino acids (aa) in length contains one Zn‑finger domain (aa 51‑91) and two phosphorylation sites at Ser168 and Ser203. There is one potential isoform that shows a deletion of aa 84‑155. Over aa 147‑228, human RNF2 shares 99% aa identity with mouse RNF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF2
• Uniprot:
  • Q99496(Human)