RNF2 Antibody (3B8) - Azide and BSA Free

Western Blot: RNF2 Antibody (3B8) [H00006045-M08] - Analysis of RNF2 expression in human placenta.

Immunocytochemistry/Immunofluorescence: RNF2 Antibody (3B8) [H00006045-M08] - Analysis of monoclonal antibody to RNF2 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: RNF2 Antibody (3B8) [H00006045-M08] - Analysis of RNF2 expression in PC-12 (Cat # L012V1).

Western Blot: RNF2 Antibody (3B8) [H00006045-M08] - Analysis of RNF2 expression in Hela S3 NE (Cat # L013V3).

Western Blot: RNF2 Antibody (3B8) [H00006045-M08] - Analysis of RNF2 expression in Raw 264.7 (Cat # L024V1).

Western Blot: RNF2 Antibody (3B8) [H00006045-M08] - Analysis of RNF2 expression in NIH/3T3 (Cat # L018V1).

ELISA: RNF2 Antibody (3B8) [H00006045-M08] - Detection limit for recombinant GST tagged RNF2 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 3B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RNF2 Antibody (3B8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RNF2 (NP_009143, 192 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQ
• Specificity: RNF2 - ring finger protein 2 (3B8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF2 Antibody (3B8) - Azide and BSA Free

• BAP-1
• BAP1RING finger protein 1B
• DING
• DINGRING finger protein BAP-1
• EC 6.3.2
• HIPI3
• HIPI3RING1BE3 ubiquitin-protein ligase RING2
• Huntingtin-interacting protein 2-interacting protein 3
• Protein DinG
• ring finger protein 2HIP2-interacting protein 3
• RING1B
• RING2
• RING2EC 6.3.2.-
• RNF2

Background

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF2
• Entrez:
  • 6045(Human)
• Uniprot:
  • NP_009143(Human)