RNF168 Antibody

Western Blot: RNF168 Antibody [H00165918-B01P] - Analysis of RNF168 expression in transfected 293T cell line by RNF168 polyclonal antibody. Lane 1: RNF168 transfected lysate(62.81 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: RNF168 Antibody [H00165918-B01P] - Analysis of purified antibody to RNF168 on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

RNF168 Antibody Summary

• Immunogen: RNF168 (NP_689830.2, 1 a.a. - 571 a.a.) full-length human protein. MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLCCPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEVADDYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLLAEEEEEEKRQAEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLNSRKSDPVTPKSEKKSKNKQRNTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK
• Specificity: Reacts with ring finger protein 168.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: RNF168
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: This antibody is reactive against transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF168 Antibody

• E3 ubiquitin-protein ligase RNF168
• EC 6.3.2
• EC 6.3.2.-
• FLJ39749
• ring finger protein 168FLJ35794
• RNF168

Background

The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF168
• Entrez:
  • 165918(Human)
• Uniprot:
  • NP_689830.2(Human)