RNF168 Antibody (3E1)

Western Blot: RNF168 Antibody (3E1) [H00165918-M01] - RNF168 monoclonal antibody (M01), clone 3E1 Analysis of RNF168 expression in HepG2.

Immunocytochemistry/Immunofluorescence: RNF168 Antibody (3E1) [H00165918-M01] - Analysis of monoclonal antibody to RNF168 on HepG2 cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: RNF168 Antibody (3E1) [H00165918-M01] - Analysis of monoclonal antibody to RNF168 on formalin-fixed paraffin-embedded human prostate cancer. Antibody concentration 1.5 ug/ml.

ELISA: RNF168 Antibody (3E1) [H00165918-M01] - Detection limit for recombinant GST tagged RNF168 is 0.03 ng/ml as a capture antibody.

Product Discontinued

RNF168 Antibody (3E1) Summary

• Immunogen: RNF168 (NP_689830, 462 a.a. ~ 571 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK
• Specificity: RNF168 (3E1)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF168
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1.5ug/mL
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. It has also been used for immunofluoresence, immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF168 Antibody (3E1)

• E3 ubiquitin-protein ligase RNF168
• EC 6.3.2
• EC 6.3.2.-
• FLJ39749
• ring finger protein 168FLJ35794
• RNF168

Background

The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repairproteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSBrepair (Stewart et al., 2009 (PubMed 19203578)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF168 Antibody (H00165918-M01)(4)

Publications using H00165918-M01

• Longarini EJ, Dauben H, Locatelli C et al. Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Molecular cell 2023-04-25 [PMID: 37116497]
• Lu X, Xu M, Zhu Q et al. RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-04-01 [PMID: 33710666] (ICC/IF, Human)
• Luczak MW, Zhitkovich A. Monoubiquitinated g-H2AX: Abundant product and specific biomarker for non-apoptotic DNA double-strand breaks. Toxicol. Appl. Pharmacol. 2018-07-10 [PMID: 30006243] (WB, Human)
• Sharma A, Alswillah T, Singh K et al. USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination Autophagy. 2018-09-19 [PMID: 29995557] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: RNF168
• Entrez:
  • 165918(Human)
• Uniprot:
  • NP_689830(Human)