RNF135 Overexpression Lysate (Native)

Images

 
Western Blot: RNF135 Overexpression Lysate (Adult Normal) [NBP2-10246] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNF135.

Product Details

Summary
Applications WB

Order Details

RNF135 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001171921. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RNF135, mRNA.
Gene
RNF135

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).

Lysate Details for RNF135

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RNF135 Overexpression Lysate (Native)

  • E3 ubiquitin-protein ligase RNF135
  • EC 6.3.2.-
  • MGC13061
  • REUL
  • ring finger protein 135RIG-I E3 ubiquitin ligase
  • Riplet

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00029956-M01A
Species: Hu, Mu
Applications: WB, ELISA, IHC-P
NBP2-20214
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
H00006124-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
H00058516-B02P
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-80104
Species: Hu
Applications: WB
NBP1-83192
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88582
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85385
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22298
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13252
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-87847
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2269
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-20216
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-76549
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC
NBP1-06069
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-81333
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-57476
Species: Hu
Applications: WB, ICC/IF, IHC
MAB7158
Species: Hu, Mu
Applications: WB, IHC
NBP2-13251
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for RNF135 Lysate (NBP2-10246) (0)

There are no publications for RNF135 Lysate (NBP2-10246).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RNF135 Lysate (NBP2-10246) (0)

There are no reviews for RNF135 Lysate (NBP2-10246). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RNF135 Lysate (NBP2-10246) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RNF135 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for RNF135 Lysate (NBP2-10246)

Discover related pathways, diseases and genes to RNF135 Lysate (NBP2-10246). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RNF135 Lysate (NBP2-10246)

Discover more about diseases related to RNF135 Lysate (NBP2-10246).
 

Pathways for RNF135 Lysate (NBP2-10246)

View related products by pathway.

PTMs for RNF135 Lysate (NBP2-10246)

Learn more about PTMs related to RNF135 Lysate (NBP2-10246).
 

Research Areas for RNF135 Lysate (NBP2-10246)

Find related products by research area.

Blogs on RNF135

There are no specific blogs for RNF135, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol RNF135

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-10246 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

RNF135 Recombinant Protein
H00084282-P01-10ug
RPL7 Antibody
NB100-2269
RPL7 Antibody
NB100-2269
RPL7 Antibody
NB100-2269
RNF135 Antibody
NBP1-81212
RNF135 Antibody
NBP1-81212
LASS2 Antibody (1A6)
H00029956-M01A
LASS2 Antibody (1A6)
H00029956-M01A
LASS2 Antibody (1A6)
H00029956-M01A