RNF114 Antibody

Western Blot: RNF114 Antibody [NBP2-97786] - Lane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa

Immunohistochemistry-Paraffin: RNF114 Antibody [NBP2-97786] - Staining of human RNF114 in human liver with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: RNF114 Antibody [NBP2-97786] - Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-RNF114 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RNF114 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :26 kDa

Product Discontinued

RNF114 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human RNF114 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF114
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 0.5-2 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for RNF114 Antibody

• PSORS12
• RNF114
• ZNF228
• ZNF313

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF114