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![Western Blot: RNF114 Antibody [NBP3-15333] - Western blot analysis of extracts of various cell lines, using RNF114 antibody (NBP3-15333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/RNF114-Antibody-Western-Blot-NBP3-15333-img0001.jpg "Western Blot: RNF114 Antibody [NBP3-15333] - Western blot analysis of extracts of various cell lines, using RNF114 antibody (NBP3-15333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: RNF114 Antibody - Azide and BSA Free [NBP3-23520] - Immunochemical staining of human RNF114 in human kidney with NBP3-23520 at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23520_rabbit-rnf114-pab-azide-and-bsa-free-136202422345071.png "Immunohistochemistry-Paraffin: RNF114 Antibody - Azide and BSA Free [NBP3-23520] - Immunochemical staining of human RNF114 in human kidney with NBP3-23520 at 1:500 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: RNF114 Antibody - Azide and BSA Free [NBP3-23520] - Immunochemical staining of human RNF114 in human liver with NBP3-23520 at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23520_rabbit-rnf114-pab-azide-and-bsa-free-136202422345022.png)
![Western Blot: RNF114 Antibody (PSH01-99) [NBP3-32910] - Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (NBP3-32910) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32910) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32910_rabbit-rnf114-mab-psh01-99-288202412442992.jpg "Western Blot: RNF114 Antibody (PSH01-99) [NBP3-32910] - Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (NBP3-32910) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32910) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")