Rit2 Antibody (3F2)

Sandwich ELISA: Rit2 Antibody (3F2) [H00006014-M01] - Detection limit for recombinant GST tagged RIT2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Rit2 Antibody (3F2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RIT2 (AAH18060, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEVENEASCSPGSASGGSREYKVVMLGAGGVGKSAMTMQFISHQFPDYHDPTIEDAYKTQVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIPLVLVGNKIDLEQFRQVSTEEGLSLAQEYNCGFFETSAALRFCIDDAFHGLVREIRKKESMPSLMEKKLKRKDCLWKKLKGSLKKKRENMT
• Localization: Plasma membrane
• Specificity: RIT2 - Ras-like without CAAX 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIT2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rit2 Antibody (3F2)

• GTP-binding protein Rit2
• GTP-binding protein Roc2
• Ras-like protein expressed in neurons
• Ras-like without CAAX 2
• Ras-like without CAAX protein 2
• RIBA
• Ric (Drosophila)-like, expressed in neurons
• Ric-like, expressed in neurons
• RIN
• Rit2
• ROC2

Background

The Ras super-family comprises a group of structurally related low molecular weight (20-30 kDa) proteins, which bind GTP or GDP and exhibit a low intrinsic GTPase activity. They are involved in signal transduction and the regulation of a variety of cellular processes, including cell growth, transformation, differentiation and morphogenesis, nucleocytoplasmic transport, and apoptosis. Ras proteins promote cancer by disrupting the normal controls of cell proliferation and differentiation. All Ras subgroups (Ras, Rho, Rab, Ran, and ADP ribosylation factor) contain five highly conserved domains (G1-G5) and act as molecular switches by alternating between an active GTP-bound form and an inactive GDP-bound form. Rit2 (Ras-like protein in neurons) and Rit1 (Ras-like protein in tissues) share 50% homology to Ras. Both contain the five highly conserved domains (G1-G5), bind GTP in vitro, and are membrane-associated. However, both lack a CAAX box and their mechanism of membrane association is distinct from the typical Ras protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIT2
• Entrez:
  • 6014(Human)
• Uniprot:
  • AAH18060(Human)