RISC Antibody

Western Blot: RISC Antibody [NBP2-97783] - Anti-RISC rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa

Immunohistochemistry: RISC Antibody [NBP2-97783] - Immunochemical staining of human RISC in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry: RISC Antibody [NBP2-97783] - Immunochemical staining of human RISC in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Product Discontinued

RISC Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human RISC fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCPEP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RISC Antibody

• EC 3.4.16
• HSCP1
• retinoid inducible serine carboxypeptidase
• retinoid-inducible serine carboxypeptidase
• RISCEC 3.4.16.-
• SCP1
• serine carboxypeptidase 1serine carboxypeptidase 1 precursor protein

Background

Vascular smooth muscle activation is a salient feature of several pathological conditions including atherosclerosis, hypertension, vein graft failure, restenosis, and transplant arteriopathy (1,2). Several new genes whose proteins could lead to such biological effects have been identified. One such protein is RISC. Tissue expression pattern of Retinoid-inducible serine carboxypeptidase (RISC) in human revealed that it was highly expressed in kidney and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCPEP1