Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (973210) [Unconjugated]

236-EG) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-Ribosomal Protein S6/RPS6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > 236-EG) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-Ribosomal Protein S6/RPS6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-Ribosomal Protein S6/RPS6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Ribosomal Protein S6/RPS6 was detected in immersion fixed MCF-7 human breast cancer cell line untreated or treated with Rapamycin using Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Ribosomal Protein S6/RPS6 was detected in immersion fixed MCF-7 human breast cancer cell line untreated or treated with Rapamycin using Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 973210
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (973210) [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human Ribosomal Protein S6 S235/S236 sites
  Accession # P62753
• Modification: p Ser235, p Ser236
• Specificity: Detects human Ribosomal Protein S6 when dually phosphorylated at S235 and S236.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (973210) [Unconjugated]

• Phosphoprotein NP33, 40S ribosomal protein S6
• pS6
• Ribosomal Protein S6
• RPS6
• S6

Background

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ribosomal Protein S6/RPS6 Antibody (MAB54361)(2)

Publications using MAB54361

• S Nemati, H Mohammad R, A Meyfour, H Pazoki, H Asadzadeh, S Shahrokh, H Mirjalali Evaluation of the mTORC activity in the presence of Toxoplasma gondii and azathioprine in human monocyte cell line BMC microbiology, 2023-03-21;23(1):77. 2023-03-21 [PMID: 36941573] (Human)
• G Chevalier, E Siopi, L Guenin-Mac, M Pascal, T Laval, A Rifflet, IG Boneca, C Demangel, B Colsch, A Pruvost, E Chu-Van, A Messager, F Leulier, G Lepousez, G Eberl, PM Lledo Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system Nature Communications, 2020-12-11;11(1):6363. 2020-12-11 [PMID: 33311466] (Western Blot, Mouse)

Bioinformatics

• Uniprot:
  • P62753()