Rhodopsin Antibody (Rho 1D4) - Chimeric - Azide and BSA Free

ELISA: Rhodopsin Antibody (Rho 1D4) - Chimeric [NBP3-09001] - Using 1D4 (Ab00337) and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Rhodopsin antibody 1D4 (Ab00337-1.1; dark blue line) and isotype control (Ab00102-1.1, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 (Pr00565, Absolute Antibody) at a concentration of 5 ug/ml. Ab02007 antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.

• Reactivity: Hu, Mu, Rt, Am, Bv, Ze
• Applications: WB, ELISA, Func, ICC/IF, IHC, IP
• Clone: Rho 1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Rhodopsin Antibody (Rho 1D4) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Rhodopsin Antibody (Rho 1D4) - Chimeric - Azide and BSA Free (NBP3-09001) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Bleached bovine rod outer segment (ROS).
• Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Radioimmunoassay
  • Western Blot
• Application Notes: This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  
  The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Rhodopsin Antibody (Rho 1D4) - Chimeric - Azide and BSA Free

• CSNBAD1
• MGC138309
• OPN2
• OPN2MGC138311
• opsin 2, rod pigment
• Opsin-2
• retinitis pigmentosa 4, autosomal dominant
• RHO
• Rhodopsin
• RP4

Background

Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHO