RHOBTB2 Antibody (DBC2/3361)

Immunohistochemistry-Paraffin: RHOBTB2 Antibody (DBC2/3361) [NBP3-13899] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Staining using RHOBTB2 antibody (DBC2/3361) at 2ug/ml in PBS for 30min RT.

Immunohistochemistry-Paraffin: RHOBTB2 Antibody (DBC2/3361) [NBP3-13899] - IHC analysis of formalin-fixed, paraffin-embedded human renal oncocytoma. Staining using RHOBTB2 antibody (DBC2/3361) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Protein Array: RHOBTB2 Antibody (DBC2/3361) [NBP3-13899] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RHOBTB2 Antibody (DBC2/3361).

• Reactivity: Hu
• Applications: IHC, MA
• Clone: DBC2/3361
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RHOBTB2 Antibody (DBC2/3361) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14286)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Human recombinant RHOBTB2 protein fragment (around aa 554-604) (exact sequence is proprietary) (Uniprot: Q9BYZ6)
• Localization: Secreted in plasm
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOBTB2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for RHOBTB2 Antibody (DBC2/3361)

• DBC2KIAA0717Deleted in breast cancer 2 gene protein
• p83
• Rho-related BTB domain containing 2
• rho-related BTB domain-containing protein 2

Background

RHOBTB2 (Rho-Related BTB Domain Containing 2) is a tumor suppressor for some forms of breast cancer and may have a role in protein transport. RHOBTB2 is known to have interactions with KDM5B, ARHGAP35, RAC2, RHOB and RHOBTB1. RHOBTB2 has been studied in relation to breast cancer, sporadic breast cancer, familial breast cancer and gastric cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOBTB2
• Uniprot:
  • Q9BYZ6(Human)