RGS2 Antibody

Images

 
Western Blot: RGS2 Antibody [NBP1-32284] - Sample (30ug whole cell lysate)A:H1299 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RGS2 Antibody [NBP1-32284] - Methanol-fixed A549, using RGS2 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RGS2 Antibody [NBP1-32284] - Paraffin-embedded rat ovary. RGS2 antibody diluted at 1:500.
Western Blot: RGS2 Antibody [NBP1-32284] - 30 ug Neuro2A whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RGS2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RGS2. The exact sequence is proprietary.
Predicted Species
Porcine (95%), Bovine (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RGS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RGS2 Antibody

  • Cell growth-inhibiting gene 31 protein
  • G0 to G1 switch regulatory 8, 24kD
  • G0/G1 switch regulatory protein 8
  • G0S8cell growth-inhibiting protein 31
  • regulator of G-protein signaling 2
  • regulator of G-protein signaling 2, 24kDa
  • regulator of G-protein signalling 2, 24kD
  • regulator of G-protein signalling 2, 24kDa

Background

Regulators of G-protein signaling (RGSs) are a protein family containing a conserved 120 amino acid domain (RGS domain), which bind directly to activated G-alpha subunits (Gai and Gaq) and act as GTPase-activating proteins (GAPs). One of these proteins, RGS2, is expressed in thyroid, heart. thyroid, and monocyte-derived DCs. The function of RGS2 as a regulator of Gq is also dependent on regulatory sequences in its amino-terminal domain that direct its localization to the plasma membrane. Recent studies have shown that RGS2 is critical for blood pressure homeostasis, and has been suggested to serve as an effector of the NO-cGMP pathway that promotes vascular relaxation based on studies of aortic rings in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RGS2
Entrez
Uniprot