RGS2 Antibody - BSA Free

Immunohistochemistry-Paraffin: RGS2 Antibody [NBP1-32284] - Rat ovary. RGS2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: RGS2 Antibody [NBP1-32284] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: RGS2 Antibody [NBP1-32284] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

22RV1 whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody (NBP1-32284) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody (NBP1-32284) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RGS2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RGS2 Antibody - BSA Free (NBP1-32284) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RGS2. The exact sequence is proprietary.
• Predicted Species: Porcine (95%), Bovine (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RGS2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RGS2 Antibody - BSA Free

• Cell growth-inhibiting gene 31 protein
• G0 to G1 switch regulatory 8, 24kD
• G0/G1 switch regulatory protein 8
• G0S8cell growth-inhibiting protein 31
• regulator of G-protein signaling 2
• regulator of G-protein signaling 2, 24kDa
• regulator of G-protein signalling 2, 24kD
• regulator of G-protein signalling 2, 24kDa

Background

Regulators of G-protein signaling (RGSs) are a protein family containing a conserved 120 amino acid domain (RGS domain), which bind directly to activated G-alpha subunits (Gai and Gaq) and act as GTPase-activating proteins (GAPs). One of these proteins, RGS2, is expressed in thyroid, heart. thyroid, and monocyte-derived DCs. The function of RGS2 as a regulator of Gq is also dependent on regulatory sequences in its amino-terminal domain that direct its localization to the plasma membrane. Recent studies have shown that RGS2 is critical for blood pressure homeostasis, and has been suggested to serve as an effector of the NO-cGMP pathway that promotes vascular relaxation based on studies of aortic rings in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RGS2
• Entrez:
  • 5997(Human)
• Uniprot:
  • P41220(Human)