RFT1 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

RFT1 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human RFT1.

  Source: E. coli

  Amino Acid Sequence: QSLCFIHRYYRRSPHRPLAGLHLSP

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: RFT1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56931.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for RFT1 Recombinant Protein Antigen

• DKFZp667J092
• FLJ25945
• putative endoplasmic reticulum multispan transmembrane protein
• requiring fifty three 1 homolog (S. cerevisiae)
• RFT1 homolog (S. cerevisiae)
• RFT1, requiring fifty three 1 homolog

Background

RFT1, also known as Protein RFT1 homolog, is a 60kDa 541 amino acid protein found in the membrane. RFT1 is believed to play a role in N-linked oligosaccharide assembly. Research is being conducted on RFT1 in relationship to congenital disorder of glycosylation and hypotonia. RFT1 has been linked to the asparagine N-linked glycosylation, metabolism of proteins and post-translational protein modification pathways where it interacts with IDI1, PSMA6, ACTB, UBC and GTF2B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RFT1