Ret Antibody (RET01)

Product Discontinued

Ret Antibody (RET01) Summary

• Immunogen: BALB/C mice were injected with recombinant protein encoding the extreme C-terminal cytoplasmic region of the human ret oncoprotein.
• Localization: Cytoplasmic
• Specificity: Clone RET01 is the same as clone 3F8 of Ret antibody. This clone was raised to the C-terminal / intra-cellular domain of the Ret protein which is present in all of its isoforms. Mutations are reported to occur upstream of this domain.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RET
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This antibody may be diluted to a titer of 1:10-1:20 in an ABC method. We suggest an incubation period of 90 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Ret Antibody (RET01)

• CDHF12MTC1
• CDHR16
• EC 2.7.10
• Hirschsprung disease 1
• Hirschsprung disease)
• MEN2A
• MEN2B
• multiple endocrine neoplasia and medullary thyroid carcinoma 1
• ret proto-oncogene
• RET transforming sequence
• Ret

Background

Ret proto-oncogene encodes a cell surface glycoprotein belonging to a member of the receptor tyrosine kinase family and is located on chromosome 10q11.2. Ret is observed in several regions of the central nervous system; in the developing cranial nerve ganglia and a subset of cells within dorsal root ganglia, in motor neurons in the spinal cord and hindbrain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ret Antibody (NB600-1521)(1)

Publication using NB600-1521

• Barrenschee M, Bottner M, Hellwig I et al. Site-specific gene expression and localization of growth factor ligand receptors RET, GFRalpha1 and GFRalpha2 in human adult colon. Cell Tissue Res 2013-11-01 [PMID: 23881409] (IHC-P, Human)
  
  Details:
  Human colon, Figs 2a-b, 3, 6

FAQs for Ret Antibody (NB600-1521). (Showing 1 - 1 of 1 FAQ).

1. I wondering if this antibody recognizes both isoforms of Ret?
   • Clone RET01 is the same as clone 3F8 of Ret antibody. This clone was raised to the C-terminal / intra-cellular domain of the Ret protein which is present in all of its isoforms. Mutations are reported to occur upstream of this domain.”

Bioinformatics

• Gene Symbol: RET
• Entrez:
  • 5979(Human)