Recombinant Rat SCF Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat SCF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Martin, F.H. et al. (1990) Cell 63:203.

  The ED₅₀ for this effect is 1-5 ng/mL.

• Source: E. coli-derived rat SCF/c-kit Ligand protein
  Gln26-Ala189, with an N-terminal Met
• Accession #: NP_068615
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Kitlg
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat SCF Protein, CF

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF), also known as c‑kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 ‑ 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 ‑ 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature rat SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O‑linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel‑dickie mutant mice (11, 12). An alternatively spliced isoform of rat SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within aa 26 ‑ 189 of the ECD (corresponding to this recombinant protein), rat SCF shares 79% and 94% aa sequence identity with human and mouse SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (13). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (14). In combinantion with G‑CSF, SCF can also reduce the accumulation of beta ‑amyloid deposits in a mouse model of Alzheimer’s disease (15).

1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599.
3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
5. Kapur, R. et al. (2002) Blood 100:1287.
6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
9. Martin, F.H. et al. (1990) Cell 63:203.
10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
13. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
14. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
15. Li, B. et al. (2011) Alzheimers Res. Ther. 3:8.

Bioinformatics

• Gene Symbol: Kitlg
• Uniprot:
  • NP_068615()