Recombinant Rat EphA5 Fc Chimera Protein, CF

Images

 
There are currently no images for EphA5 (541-A5).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Rat EphA5 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat EphA5 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2) with a linear range of 0.16-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived rat EphA5 protein
Rat EphA5
Ser58-Gln573
(Asp170Glu)
Accession #P54757
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser58
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Epha5
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
84 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110 kDa, reducing conditions
Publications
Read Publication using
541-A5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat EphA5 Fc Chimera Protein, CF

  • Bsk
  • Cek7
  • EC 2.7.10
  • EC 2.7.10.1
  • Ehk1
  • EHK-1
  • EHK1Hek7
  • EK7
  • EPH homology kinase 1
  • Eph homology kinase-1
  • EPH receptor A5
  • EphA5
  • EPH-like kinase 7
  • ephrin type-A receptor 5
  • Hek7
  • receptor protein-tyrosine kinase HEK7
  • Rek7
  • TYRO4HEK7CEK7
  • tyrosine-protein kinase receptor EHK-1

Background

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA5 has been shown to bind ephrin-A5, ephrin-A1,
ephrin-A2, ephrin-A3, and ephrin-A4 (2, 3). The extracellular domains of rat EphA5 share 98.5% amino acid identity with the mouse homolog and 96.5% identity with the human homolog. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Customers Who Viewed This Item Also Viewed...

374-EA
Species: Hu
AF3034
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NBP2-02810
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF641
Species: Mu
Applications: WB, IHC
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
359-EA
Species: Hu
NBP1-47839
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-47761
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
640-A3
Species: Mu
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF611
Species: Mu
Applications: WB
NBP2-13964
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF454
Species: Mu
Applications: WB
NBP2-67327
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC

Publications for EphA5 (541-A5)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for EphA5 (541-A5) (0)

There are no reviews for EphA5 (541-A5). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for EphA5 (541-A5) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Ephrin-A5
374-EA-200
Ephrin-A1
602-A1-200

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rat EphA5 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Epha5
Entrez
Uniprot