Recombinant Rat CSMD1 Protein, CF

• Reactivity: Rt
• Format: Carrier-Free

Recombinant Rat CSMD1 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived rat CSMD1 protein
  Ala2081-Ser3170, with a C-terminal 6-His tag
• Accession #: NP_001032404
• N-terminal Sequence: No results obtained. Ala2081 predicted
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 119 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 138-173 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CSMD1 Protein, CF

• CSMD1
• CSMD-1
• CUB and sushi domain-containing protein 1
• CUB and sushi multiple domains protein 1
• KIAA1890
• Mdcr1
• PPP1R24
• UNQ5952/PRO19863

Background

Cub and sushi multiple domains-1 (CSMD1), also known as multiple domain complement regulator 1, is an approximately 388-kDa member of the neuronal cub and sushi multiple domains family that plays a role in the regulation of inflammation and complement activation (1). It may also act as a tumor suppressor and be involved in growth cone functions such as amoeboid motility and cell-cell interaction (2, 3). This 3535 amino acid (aa) transmembrane protein is highly expressed in the central nervous system and epithelial tissues. The rat CSMD1 extracellular domain (ECD) consists of 14 N-terminal cub domains that alternate with sushi domains, followed by 15 C-terminal sushi domains (1). Within the ECD, rat CSMD1 shares 93% and 98% sequence identity with human and mouse CSMD1, respectively.

1. Kraus, D. et al. (2006) J. Immunol. 176:4419.
2. Ma, C. et al. (2009) Cancer Biol. Ther. 8:907.
3. Kamal, M. et al. (2010) Breast Cancer Res. Treat. 121:555.

Bioinformatics

• Uniprot:
  • NP_001032404()