Recombinant Mouse Sulfamidase/SGSH Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Enzyme Activity

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Recombinant Mouse Sulfamidase/SGSH Protein, CF Summary

Details of Functionality
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is >0.4 pmol/min/µg, as measured under the described conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Sulfamidase/SGSH protein
Arg23-Leu502, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60 kDa, reducing conditions
Read Publications using
2969-SU in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 50 mM MES, pH 5.5
  • Recombinant Mouse Sulfamidase/SGSH (mSGSH) (Catalog # 2969-SU)
  • Substrate: 4-Nitrocatechol Sulfate (PNCS) (Sigma, Catalog # N-7251), 100 mM stock in deionized water
  • Sodium Hydroxide (NaOH) (Sigma, Catalog # 221465), 2 M stock in deionized water
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rmSGSH to 40 µg/mL in Assay Buffer.
  2. Dilute Substrate to 20 mM in Assay Buffer.
  3. Mix 150 µL of 40 µg/mL of rmSGSH and 150 µL of 20 mM of Substrate. Include a Substrate Blank with 150 µL of Assay Buffer and 150 µL of Substrate.
  4. Incubate at 37 °C for 72 hours.
  5. Stop reaction by adding 300 µL of 0.2 M NaOH to the reaction vials.
  6. Load in a plate 200 µL in duplicate from each reaction vial.
  7. Read at 510 nm (absorbance) in endpoint mode.
  8. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard P-Nitrocatechol (PNC) (Sigma, Catalog # N15553).

Per Well:
  • rmSGSH: 2 µg
  • Substrate: 5 mM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Sulfamidase/SGSH Protein, CF

  • heparan sulfate sulfatase
  • HSS
  • HSSN-sulphoglucosamine sulphohydrolase
  • MPS3A
  • MPS3Asulphamidase
  • N-sulfoglucosamine sulfohydrolase
  • SFMD
  • SGSH
  • Sulfamidase
  • Sulfoglucosamine sulfamidase
  • Sulphamidase


Also known as N-sulfoglucosamine sulfohydrolase and heparan N-sulfatase, Sulfamidase/SGSH is an important member of the sulfatase family involved in the degradation of heparan sulfate (1). The SGSH deficiency results in mucopolysaccharidosis type IIIA (MPS IIIA, Sanfilippo A syndrome), an autosomal recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations (2). The deduced amino acid sequence of mouse SGSH consists of a signal peptide (residues 1-22) and a mature chain (residues 23-502) (1). Recombinant Mouse Sulfamidase/SGSH corresponds to the mature chain and has sulfatase activity as described in the Activity section.

  1. Diez-Roux, G. and Ballabio, A. (2005) Annu. Rev. Genomics Hum. Genet. 6:355.
  2. Gliddon, B.L. et al. (2004) Mol. Genet. Metab. 83:239.

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Publications for Sulfamidase/SGSH (2969-SU)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.

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Gene Symbol Sgsh