Recombinant Mouse Siglec-E Fc Chimera Protein, CF

When Recombinant Mouse Siglec-E Fc Chimera (Catalog # 5806-SL) is immobilized at 2 μg/mL (100 μL/well), Recombinant Mouse Galectin-3 (HEK293-expressed) Protein (Catalog # 9039-GAB) binds with an ED50 of 80-800 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Siglec-E Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec‑E Fc Chimera is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Mouse Galectin-3 (HEK293-expressed) Protein (Catalog # 9039-GAB) with an ED₅₀ of 0.08-0.8 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Siglec-E protein
  

  Mouse Siglec-E (Met1-Phe355) Accession # AAH23280	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: AAH23280
• N-terminal Sequence: No results obtained, N-sequencing might be blocked: Gln20 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Siglece
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 64.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 75-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Siglec-E Fc Chimera Protein, CF

• CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5
• SiglecE
• Siglec-E

Background

Siglecs are sialic acid specific I‑type lectins that are characterized by an extracellular domain (ECD) with an N‑terminal Ig‑like V‑set domain followed by varying numbers of Ig‑like C2‑set domains (1, 2). Mouse Siglec‑E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 ‑ 85 kDa member of the CD33‑related subfamily of Siglecs. It consists of a 335 amino acid (aa) ECD with one Ig‑like V‑set domain and two Ig‑like C2‑set domains, a 21 aa transmembrane segment, and a 93 aa cytoplasmic domain that contains two immunoreceptor tyrosine‑based inhibitory motifs (ITIM) (3, 4). Rodent and primate Siglec gene families have significantly diverged, and Siglec‑9 is the most likely human ortholog of mouse Siglec‑E (1). Within the ECD, mouse Siglec‑E shares 56% and 80% aa sequence identity with human Siglec‑9 and rat Siglec‑E, respectively. Siglec‑E is expressed as a heavily N‑glycosylated disulfide‑linked homodimer and shows binding preference for disialic acids in the alpha 2‑8 linkage (3, 5). It is expressed on the surface of several hematopoietic cell types including neutrophils, NK cells, monocytes, peritoneal macrophages and B1 cells, and splenic myeloid dendritic cells and marginal zone B cells (5). Tyrosine phosphorylation of the cytoplasmic ITIMs mediates the association of Siglec‑E with the phosphatases SHP‑1 and SHP‑2 (3, 4). Siglec‑E is up‑regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists (6). Siglec‑E signaling negatively regulates the LPS‑induced production of TNF‑ alpha and IL‑6 by macrophages (4, 6). Its up‑regulation in macrophages parallels the development of endotoxin tolerance (6). Siglec‑E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL‑12 p40 production (7).

1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
3. Yu, Z. et al. (2001) Biochem. J. 353:483.
4. Ulyanova, T. et al. (2001) J. Biol. Chem. 276:14451.
5. Zhang, J.Q. et al. (2004) Eur. J. Immunol. 34:1175.
6. Boyd, C.R. et al. (2009) J. Immunol. 183:7703.
7. Erdmann, H. et al. (2009) Cell. Microbiol. 11:1600.

Publications for Siglec-E (5806-SL)(6)

Publications using 5806-SL

• He, J;Zhang, F;Wu, B;Yu, W; ST8SIA6 Sialylates CD24 to Enhance Its Membrane Localization in BRCA Cells 2024-12-26 [PMID: 39791710] (Immunocytochemistry, Mouse)
• Borges, TJ;Lima, K;Murshid, A;Lape, IT;Zhao, Y;Rigo, MM;Lang, BJ;Siddiqui, SS;Hui, E;Riella, LV;Bonorino, C;Calderwood, SK; Innate extracellular Hsp70 inflammatory properties are mediated by the interaction of Siglec-E and LOX-1 receptors bioRxiv : the preprint server for biology 2023-12-04 [PMID: 38106019] (ELISA Capture, N/A)
• Egan, H;Treacy, O;Lynch, K;Leonard, NA;O'Malley, G;Reidy, E;O'Neill, A;Corry, SM;De Veirman, K;Vanderkerken, K;Egan, LJ;Ritter, T;Hogan, AM;Redmond, K;Peng, L;Che, J;Gatlin, W;Jayaraman, P;Sheehan, M;Canney, A;Hynes, SO;Kerr, EM;Dunne, PD;O'Dwyer, ME;Ryan, AE; Targeting stromal cell sialylation reverses T cell-mediated immunosuppression in the tumor microenvironment Cell reports 2023-05-05 [PMID: 37167967] (Flow Cytometry, Mouse)
• JV Pluvinage, MS Haney, BAH Smith, J Sun, T Iram, L Bonanno, L Li, DP Lee, DW Morgens, AC Yang, SR Shuken, D Gate, M Scott, P Khatri, J Luo, CR Bertozzi, MC Bassik, T Wyss-Coray CD22 blockade restores homeostatic microglial phagocytosis in ageing brains Nature, 2019-04-03;0(0):. 2019-04-03 [PMID: 30944478] (Flow Cytometry, Mouse)
• Hsu F, Belmonte P, Constans M, Chen M, McWilliams D, Hiebert S, Shapiro V Histone Deacetylase 3 Is Required for T Cell Maturation. J Immunol, 2015-07-10;195(4):1578-90. 2015-07-10 [PMID: 26163592] (Flow Cytometry, Mouse)
• Chen , Guo-Yun, Brown , Nicholas, Wu , Wei, Khedri , Zahra, Yu , Hai, Chen , Xi, van de Vlekkert , Diantha, D'Azzo , Alessand, Zheng , Pan, Liu , Yang Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1. Elife, 2014-09-03;3(0):e04066. 2014-09-03 [PMID: 25187624] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Siglece
• Uniprot:
  • AAH23280()