Recombinant Mouse sFRP-4 Protein, CF

Recombinant Mouse sFRP‑4 (Catalog # 9806‑SF) activates Wntinduced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50for this effect is 0.2‑1.2 μg/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse sFRP-4 Protein, CF Summary

• Details of Functionality: Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.2-1.2 ug/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
• Source: Mouse myeloma cell line, NS0-derived mouse sFRP-4 protein
  Ala22-Ser351
• Accession #: Q9Z1N6
• N-terminal Sequence: Ala22
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 47-63 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse sFRP-4 Protein, CF

• DDC-4
• Frizzled protein, human endometrium
• FRP-4
• FRP-AP
• frpHE
• FRPHEMGC26498
• FrzB2
• secreted frizzled-related protein 4
• secreted frizzled-related protein 4; secreted frizzled-related protein 4
• sFRP4
• sFRP-4

Background

Secreted Frizzled Related Protein-4 (sFRP-4), also known as DDC-4, FrpAP, frpHE and FrzB-2, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled receptors (1, 2). The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Of all the sFRPs, sFRP-4 is most closely related to sFRP-3 (1). Mature mouse sFRP-4 shares 92% and 96% amino acid (aa) sequence identity with human and rat sFRP-4, respectively. sFRP-4 is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, its role in cancer is likely to be tissue dependent (6). In addition, sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (8).

1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
2. Wolf, V. et al. (1997) FEBS Letters 417:385.
3. Drake, J.M. et al. (2003) Apoptosis 8:389.
4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
6. Abu-Jawdeh, G. et al. (1999) Lab Invest. 79:439.
7. Wong, S.C. et al. (2002) J. Pathol. 196:145.
8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.

Publications for sFRP-4 (9806-SF)(2)

Publications using 9806-SF

• K Chen, PY Ng, R Chen, D Hu, S Berry, R Baron, F Gori Sfrp4 repression of the Ror2/Jnk cascade in osteoclasts protects cortical bone from excessive endosteal resorption Proc. Natl. Acad. Sci. U.S.A., 2019-06-25;0(0):. 2019-06-25 [PMID: 31239337] (Bioassay, Mouse, Transgenic Mouse)
• H Guo, Y Xing, F Deng, K Yang, Y Li Secreted Frizzled-related protein 4 inhibits the regeneration of hair follicles PeerJ, 2019-01-04;6(0):e6153. 2019-01-04 [PMID: 30631645] (In Vivo, Mouse)

Bioinformatics

• Uniprot:
  • Q9Z1N6()