Recombinant Mouse sFRP-4 Protein, CF

Images

 
Recombinant Mouse sFRP‑4 (Catalog # 9806‑SF) activates Wntinduced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50for this effect is 0.2‑1.2 μg/mL in the presence of 5 ng/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse sFRP-4 Protein, CF Summary

Details of Functionality
Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.2-1.2 ug/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Mouse myeloma cell line, NS0-derived mouse sFRP-4 protein
Ala22-Ser351
Accession #
N-terminal Sequence
Ala22
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
47-63 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse sFRP-4 Protein, CF

  • DDC-4
  • Frizzled protein, human endometrium
  • FRP-4
  • FRP-AP
  • frpHE
  • FRPHEMGC26498
  • FrzB2
  • secreted frizzled-related protein 4
  • secreted frizzled-related protein 4; secreted frizzled-related protein 4
  • sFRP4
  • sFRP-4

Background

Secreted Frizzled Related Protein-4 (sFRP-4), also known as DDC-4, FrpAP, frpHE and FrzB-2, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled receptors (1, 2). The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Of all the sFRPs, sFRP-4 is most closely related to sFRP-3 (1). Mature mouse sFRP-4 shares 92% and 96% amino acid (aa) sequence identity with human and rat sFRP-4, respectively. sFRP-4 is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, its role in cancer is likely to be tissue dependent (6). In addition, sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (8).
  1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
  2. Wolf, V. et al. (1997) FEBS Letters 417:385.
  3. Drake, J.M. et al. (2003) Apoptosis 8:389.
  4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
  5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
  6. Abu-Jawdeh, G. et al. (1999) Lab Invest. 79:439.
  7. Wong, S.C. et al. (2002) J. Pathol. 196:145.
  8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.

Customers Who Viewed This Item Also Viewed...

1169-FR/CF
Species: Mu
2629-FG/CF
Species: Mu
NBP2-20331
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
1341-WF/CF
Species: Hu
NBP2-35212
Species: Hu
AF1118
Species: Hu
Applications: WB, IHC
AF6150
Species: Mu
Applications: WB
NBP1-86713
Species: Hu
Applications: IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
7584-SF/CF
Species: Hu
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-66987
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24695
Species: Hu
Applications: WB, IHC-P
9806-SF
Species: Mu
Applications: Bioactivity

Publications for sFRP-4 (9806-SF) (0)

There are no publications for sFRP-4 (9806-SF).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for sFRP-4 (9806-SF) (0)

There are no reviews for sFRP-4 (9806-SF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for sFRP-4 (9806-SF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

sFRP-4 Antibody
NBP1-81866
Dkk-1 [Unconjugated]
5439-DK-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse sFRP-4 Protein, CF and receive a gift card or discount.

Bioinformatics

Entrez
Uniprot