Recombinant Mouse SECTM1A Fc Chimera Protein, CF

When Recombinant Recombinant Mouse CD7 (Catalog # 8145-CD) is immobilized at 1 µg/mL, Recombinant Mouse SECTM1A Fc Chimera (Catalog # 7837-ST) binds with an ED50 of 30‑150 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SECTM1A Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD7 (Catalog # 8145-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Mouse SECTM1A Fc Chimera that produces 50% of the optimal binding response is 30-150 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse SECTM1A protein
  

  Mouse SECTM1A (Gln28-Thr165) Accession # NP_663348	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_663348
• N-terminal Sequence: Gln28 predicted: No results obtained, sequencing might be blocked
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sectm1a
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 42.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-66 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SECTM1A Fc Chimera Protein, CF

• K12
• SECTM
• SECTM1A

Background

SECTM1A (secreted and transmembrane 1A), is 192 amino acid (aa) protein that shares approximately 45‑51%, 81%, and 43% aa sequence identity with human SECTM1 (also called K12), rat SECTM1, and mouse SECTM1B/K12, respectively, and appears to share structural and functional characteristics with other SECTM1 proteins. Human SECTM1 can be found either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form, and is produced by some myeloid cells and by thymic epithelia and fibroblasts (1‑3). Stimulation with IFN‑ gamma is often necessary to detect human SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Mouse SECTM1A cDNA encodes a signal sequence, an extracellular domain with four potential N‑linked glycosylation sites, a transmembrane sequence, and a very short (approximately 6 aa) cytoplasmic sequence (6). SECTM1 proteins from human and mouse show species‑specific binding of CD7 and co‑stimulation of T cells, including enhancement of CD3‑induced proliferation (2‑4).

1. Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.
2. Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.
3. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
4. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
5. Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.
6. Swiss-Prot Accession # Q921W8.

Publications for SECTM1A (7837-ST)(1)

Publication using 7837-ST

• X Mu, P Wang, X Wang, Y Li, H Zhao, Q Li, K Essandoh, S Deng, T Peng, GC Fan Identification of a Novel Antisepsis Pathway: Sectm1a Enhances Macrophage Phagocytosis of Bacteria through Activating GITR J. Immunol., 2020-08-07;0(0):. 2020-08-07 [PMID: 32769121] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Sectm1a
• Uniprot:
  • NP_663348()