Recombinant Mouse Plexin B2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Plexin B2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA.

  When Recombinant Mouse Plexin B2 is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera (Catalog # 5235-S4) binds with an apparent K_D < 10 nM.

  Optimal dilutions should be determined by each laboratory for each applications.

• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Plexin B2 protein
  

  Mouse Plexin B2 (Leu20 - Trp1029) Accession # NP_001152993	HPGGGSGGGSGGGS	6-His tag
  N-terminus		C-terminus

• Accession #: NP_001152993
• N-terminal Sequence: Leu20
• Protein/Peptide Type: Recombinant Proteins
• Gene: Plxnb2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-155 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Plexin B2 Protein, CF

• MM1
• MM1KIAA0315dJ402G11.3
• Nbla00445
• PLEXB2
• Plexin B2
• plexin-B2
• PLXNB2

Background

Plexin B2 is a 240 kDa type I transmembrane (TM) glycoprotein of the Plexin B family of semaphorin receptors (1, 2). The mouse Plexin B2 cDNA encodes 1842 amino acids (aa) that include a 19 aa signal sequence, a 1182 aa extracellular domain (ECD), a 21 aa TM domain, and a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20 ‑ 468) and three IPT repeats (aa 806 ‑ 1096). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20 ‑ 1168) and an 80 kDa TM beta ‑chain, that remain noncovalently linked (1). Multiple splice variants may exist. Within aa 20 ‑ 1029 in the ECD, mouse Plexin B2 shares 82%, 93%, 80% and 79% aa identity with human, rat, canine and bovine Plexin B2, respectively. The B Plexins (B1, B2 and B3) share approximately 40% aa identity with each other. Plexin B2 mRNA is expressed in proliferating cerebellar granule cell progenitors, neuroepithelium, developing neurons, growth plate chondrocytes, tooth bud inner enamel epithelium, glomeruli and mesenchyme of the developing kidney, and in germinal center B lymphocytes when T cell help is present (3 ‑ 7). Plexin B2 is often co‑expressed with Plexin B1, and the two may form heterodimers (1, 4, 6). Genetic deletion of mouse Plexin B2 results in defects in proliferation and migration of cerebellar granule cells, abnormal development of the neural tube and disorganization of the embryonic brain; these defects are not seen when Plexin B1 is deleted (8 - 10). In adults, Plexin B2 is expressed in specialized vascular endothelia, pancreatic islets of Langerhans, and adrenal glands (11). Plexin B2 serves as a receptor for type 4 semaphorins, especially Sema4C and Sema4G (8 ‑ 12). B Plexins, including Plexin B2, can bind the scatter factor receptors, Met and Ron, and activate them upon semaphorin engagement (1, 13).

1. Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
2. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
3. Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
4. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
5. Zhang, M. et al. (2008) Bone 43:511.
6. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
7. Yu, D. et al. (2008) Immunol. Cell Biol. 86:3.
8. Deng, S. et al. (2007) J. Neurosci. 27:6333.
9. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
10. Maier, V. et al. (2011) Mol. Cell. Neurosci. 46:419.
11. Zielonka, M. et al. (2010) Exp. Cell Res. 316:2477.
12. Yukawa, K. et al. (2010) Int. J. Mol. Med. 25:225.
13. Conrotto, P. et al. (2004) Oncogene 23:5131.

Publications for Plexin B2 (6836-PB)(2)

Publications using 6836-PB

• Borrelli, C;Roberts, M;Eletto, D;Hussherr, MD;Fazilaty, H;Valenta, T;Lafzi, A;Kretz, JA;Guido Vinzoni, E;Karakatsani, A;Adivarahan, S;Mannhart, A;Kimura, S;Meijs, A;Baccouche Mhamedi, F;Acar, IE;Handler, K;Ficht, X;Platt, RJ;Piscuoglio, S;Moor, AE; In vivo interaction screening reveals liver-derived constraints to metastasis Nature 2024-07-24 [PMID: 39048831] (Bioassay, Mouse)
• Chen Jiang, Ahsan Javed, Laura Kaiser, Michele M. Nava, Rui Xu, Dominique T. Brandt et al. Mechanochemical control of epidermal stem cell divisions by B-plexins Nature Communications 2021-02-26 [PMID: 33637728] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Plxnb2
• Uniprot:
  • NP_001152993()