Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125. When 0.25 µg/mL (100 µL/well) of rmOsteoactivin/Fc Chimera is immobilized on goat anti-mouse Fc Chimera antibody coated wells, it will support >30% adhesion of added SVEC4-10 cells (1 x 10⁵ cells/well).
• Source: Mouse myeloma cell line, NS0-derived mouse Osteoactivin/GPNMB protein
  

  Mouse Osteoactivin (Lys23 - Asn502) Accession # Q8BVA0	IEGRMDP	Mouse IgG2A (Glu98 - Lys330)
  N-terminus		C-terminus

• Accession #: Q8BVA0
• N-terminal Sequence: Lys23
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Gpnmb
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 80.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF

• DC-HIL
• glycoprotein (transmembrane) nmb
• glycoprotein NMB
• GPNMB
• HGFIN
• HGFINglycoprotein nmb-like protein
• NMBosteoactivin
• Osteoactivin
• Transmembrane glycoprotein HGFIN
• transmembrane glycoprotein NMB

Background

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.

Publications for Osteoactivin/GPNMB (2330-AC)(2)

Publications using 2330-AC

• Hideaki Hara Glycoprotein nonmetastatic melanoma protein B (GPNMB) promotes the progression of brain glioblastoma via Na(+)/K(+)-ATPase Biochem. Biophys. Res. Commun., 2016-11-09;481(2):7-12. 2016-11-09 [PMID: 27836549] (Bioassay, Mouse)
• Carmi Y, Spitzer M, Linde I, Burt B, Prestwood T, Perlman N, Davidson M, Kenkel J, Segal E, Pusapati G, Bhattacharya N, Engleman E Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity. Nature, 2015-04-29;521(7550):99-104. 2015-04-29 [PMID: 25924063] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Gpnmb
• Uniprot:
  • Q8BVA0()