Recombinant Mouse Limitin/IFN-zeta Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Limitin/IFN-zeta Protein Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 3-15 pg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Limitin/IFN-zeta protein
  Leu22-Arg182, with a C-terminal Ile and 6-His tag
• Accession #: BAA83749
• N-terminal Sequence: Leu22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ifnz
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Limitin/IFN-zeta Protein

• 6030405N23Rik
• BGIF
• Ifnz
• IFN-z
• IFNzeta
• interferon zeta
• Limitin
• Lmtn

Background

Limitin is a secreted interferon (IFN)-like glycoprotein. It has antiviral activity and selectively inhibits B-lineage lymphoid-cell development both in vivo and in vitro, while having little effect on myelopoiesis or erythropoiesis. Mouse limitin cDNA encodes a 182 aa residue precursor protein with an N-terminal 21 aa residue putative signal peptide. Limitin binds to the IFN-alpha / beta receptors and induces IFN regulatory factor-1. Mouse limitin displays 32%, 26% and 30% amino acid identity with mouse IFN-alpha , mouse IFN-beta , and human IFN-omega , respectively (1 - 2).

1. Oritani, K. et al. (2001) J. Mol. Med. 79:168.
2. Oritani, K. et al. (2000) Nature Med. 6:659.

Bioinformatics

• Gene Symbol: Ifnz
• Uniprot:
  • BAA83749()