Recombinant Mouse Layilin Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Layilin Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmLayilin at 5 µg/mL (100 µL/well) can bind biotinylated hyaluronan with a linear range of 0.8-50 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Layilin protein
  Lys18-Glu229 (Ala29-Gly36 del), with a C-terminal 10-His tag
• Accession #: Q8C351.3
• N-terminal Sequence: Lys18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Layn
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 24.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Layilin Protein, CF

• FLJ30977
• FLJ31092
• Layilin
• LAYN
• LYAN

Background

Layilin (named after the L-A-Y-I-L-I amino acid motif in the transmembrane segment) is a member of the animal C-type lectin family (1). The mouse Layilin cDNA encodes a 402 amino acid (aa) type I transmembrane protein that, based on the orthologous molecule in hamster, contains a 19 aa signal sequence, a 211 aa extracellular domain (ECD), a 30 aa transmembrane segment, and a 141 aa cytoplasmic tail (2, 3). The ECD of mouse Layilin shares 85%, 70%, and 77% aa sequence identity with that of hamster, rat, and human Layilin, respectively. The ECD includes one carbohydrate recognition domain that contains a single potential Ca⁺⁺-binding site (3). The intracellular region contains three types of LH (Layilin Homology) repeats: three 16 - 18 aa LH1 repeats, three 5 aa LH2 repeats, and one 4 aa LH3 repeat (3). Adjacent LH2-LH3 tandem arrays provide docking sites for the actin cytoskeleton adaptor proteins talin, merlin, and radixin (3, 4, 5). Layilin interacts with the ubiquitous extracellular matrix component hyaluronan but not with heparin, chondroitin sulfate, or N-acetyl glucosamine (6). Layilin is widely expressed and localizes to ruffling edges in spreading and migrating cells (3, 5). It does not localize to focal contacts and does not interact with the focal contact proteins alpha ‑actinin, vinculin, tensin, paxillin, tubulin, or Integrin  beta 1 (3).

1. McGreal, E.P. et al. (2004) Mol. Immunol. 41:1109.
2. Genbank Accession # XP_146887.
3. Borowsky, M.L. and R.O. Hynes (1998) J. Cell Biol. 143:429.
4. Rees, D.J.G. et al. (1990) Nature 347:685.
5. Bono, P. et al. (2005) Exp. Cell Res. 308:177.
6. Bono, P. et al. (2001) Mol. Biol. Cell 12:891.

Review for Layilin (2646-LA) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer Binding assay/Protein-protein interaction 01/13/2020

Summary

• Application: Binding assay/Protein-protein interaction
• Lot: NBY0616121
• Comments: For the kind method to meet custumor's need

Bioinformatics

• Gene Symbol: Layn
• Uniprot:
  • Q8C351.3()