Recombinant Mouse Integrin alpha 9 beta 1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha 9 beta 1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. The ED 50 for this effect is typically 0.6-3 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 9 beta 1 protein

  Mouse Integrin alpha 9 (Tyr31-Val979) Accession # CAC69080

  His-Pro

  GGGSGGGS

  Acidic Tail

  HHHHHH

  Mouse Integrin beta 1 (Gln21-Asp728) Accession # P09055

  His-Pro

  GGGSGGGS

  Basic Tail

  N-terminus

  C-terminus

• Accession #: CAC69080 (Integrin alpha 9) & P09055 (Integrin beta 1)
• N-terminal Sequence: Tyr31 (Integrin alpha 9) & Gln21 predicted: No results obtained, sequencing might be blocked (Integrin beta 1)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Itga9
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 114 kDa (Integrin alpha 9) & 86.5 kDa (Integrin beta 1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-160 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha 9 beta 1 Protein, CF

• Integrin alpha 9 beta 1

Background

Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit (1-3). It is the non-covalent heterodimer of 150 kDa alpha 9 and 130 kDa beta 1 type I transmembrane glycoprotein subunits (3). The alpha 9 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (2). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 9 beta -propeller to form a binding domain when the dimer is in the active, extended and open conformation. The 1006 amino acid (aa) mouse alpha 9 extracellular domain (ECD) shares 95% and 89% aa sequence identity with rat and human alpha 9, respectively, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 93-94% with human, bovine, porcine, ovine, canine and feline beta 1. Each subunit has a transmembrane sequence and a short cytoplasmic tail which, for alpha 9, contains binding sites for paxillin and SSAT (spermine/spermidine-N-acetyltransferase) (4, 5). Integrin alpha 9 beta 1 inhibits cell spreading via signaling through paxillin (4). SSAT interaction with alpha 9 beta 1 allows catalysis that lifts spermine/spermidine inhibition of KIR potassium channels, and is shown to enhance cell migration or suppress airway smooth muscle contraction (5, 6). Lymphangiogenic vascular endothelia growth factors VEGF-C and VEGF-D are ligands for Integrin alpha 9 beta 1 (7, 8) Genetic deletion of mouse alpha 9 beta 1 results in a fatal chylothorax due to abnormal lymphatic development (9). Integrin alpha 9 beta 1 interacts in cis with G-CSF R and enhances its signals, contributing to granulopoiesis (10). It also binds VCAM-1, which promotes neutrophil transendothelial migration and delays apoptosis (11, 12). Other ligands for alpha 9 beta 1 include polydom/SVEP1, tenascin, osteopontin, nerve growth factor, and most members of the ADAM family (13-16).

1. Gupta, S.K. and N.E. Vlahakis (2010) Cell Adh. Migr. 4:194. 
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Palmer, E. L. et al. (1993) J. Cell Biol. 123:1289.
4. Liu, S. et al. (2001) J. Biol. Chem. 276:37086.
5. deHart, G. W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:7188.
6. Chen, C. et al. (2012) J. Clin. Invest. 122:2916.
7. Vlahakis, N. E. et al. (2005) J. Biol. Chem. 280:4544.
8. Vlahakis, N. E. et al. (2007) J. Biol. Chem. 282:15187.
9. Huang, X. Z. et al. (2000) Mol. Cell. Biol. 20:5208.
10. Chen, C. et al. (2006) Immunity 25:895.
11. Ross, E. A. et al. (2006) Blood 107:1178.
12. Taooka, Y. et al. (1999) J. Cell Biol. 145:413.
13. Sato-Nishiuchi, R. et al. (2012) J. Biol. Chem. 287:25615.
14. Marcinkiewicz, C. et al. (2000) J. Biol. Chem. 275:31930.
15. Staniszewska, I. et al. (2008) J. Cell Sci. 121:504.
16. Eto, K. et al. (2002) J. Biol. Chem. 277:17804.

Publications for Integrin alpha 9 beta 1 (7826-A9)(1)

Publication using 7826-A9

• N Matsumoto, S Kon, T Nakatsuru, T Miyashita, K Inui, K Saitoh, Y Kitai, R Muromoto, JI Kashiwakur, T Uede, T Matsuda A Novel ?9 Integrin Ligand, XCL1/Lymphotactin, Is Involved in the Development of Murine Models of Autoimmune Diseases J. Immunol., 2017-05-26;0(0):. 2017-05-26 [PMID: 28550205] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Itga9
• Uniprot:
  • CAC69080 (Integrin alpha 9) & P09055 (Integrin beta 1)()