Recombinant Mouse IL-28B/IFN-lambda 3 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse IL-28B/IFN-lambda 3 Protein Summary

• Details of Functionality: Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED₅₀ for this effect is 0.800-8.00 ng/mL.
• Source: E. coli-derived mouse IL-28B/IFN-lambda 3 protein
  Asp20-Val193, with an N-terminal Met
• Accession #: NP_796370
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ifnl3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-28B/IFN-lambda 3 Protein

• interleukin-28B
• IFN-lambda 3
• IL28B
• IL-28B
• IL28C
• interferon, lambda 3

Background

Human IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and the type I IFN family (15 - 19% aa sequence identity) (1 - 3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor  alpha (IL-28 R alpha , also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Mouse IL-28B cDNA encodes a 193 amino acid residue precursor protein with a putative 15 aa signal peptide. It shares 61%, 62% and 52% aa sequence identity with human IL-28A, human IL-28B and human IL-29, respectively.

1. Vilcek, J. (2003) Nature Immunol. 4:8.
2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

Publications for IL-28B/IFN-lambda 3 (1789-ML)(10)

Publications using 1789-ML

• Danastas, K;Guo, G;Merjane, J;Hong, N;Larsen, A;Miranda-Saksena, M;Cunningham, AL; Interferon inhibits the release of herpes simplex virus-1 from the axons of sensory neurons mBio 2023-09-01 [PMID: 37655893] (Bioassay, Rat)
• J Won, A Jo, CH Gil, S Kim, H Shin, H Jik Kim Inhaled delivery of recombinant interferon-lambda restores allergic inflammation after development of asthma by controlling Th2- and Th17-cell-mediated immune responses International immunopharmacology, 2022-08-27;112(0):109180. 2022-08-27 [PMID: 36030690] (In Vivo, Mouse)
• Z Li, J Wang, C Chen, Q He, X Xu, Z Da, B Wang, M Wang, X Gao, G Zhang, Q Gao, X Si, Y Luo, X Ma Intratumoral IL-28B Gene Delivery Elicits Antitumor Effects by Remodeling of the Tumor Microenvironment in H22-Bearing Mice Journal of Immunology Research, 2022-07-28;2022(0):1345971. 2022-07-28 [PMID: 35935577] (Cell Culture, Mouse)
• JJ McGuire, JS Frieling, CH Lo, T Li, A Muhammad, HR Lawrence, NJ Lawrence, LM Cook, CC Lynch Mesenchymal stem cell-derived interleukin-28 drives the selection of apoptosis resistant bone metastatic prostate cancer Nature Communications, 2021-02-01;12(1):723. 2021-02-01 [PMID: 33526787] (Bioassay, Human)
• AT Liou, CC Liao, SF Chou, YS Chang, CS Chang, C Shih Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model J. Biomed. Sci., 2019-11-11;26(1):93. 2019-11-11 [PMID: 31711481] (In Vivo, Mouse)
• Yuwen Zhang, Jiaqing Hao, Jun Zeng, Qiang Li, Enyu Rao, Yanwen Sun et al. Epidermal fatty acid binding protein prevents chemical-induced skin tumorigenesis by regulation of TPA-induced IFN/p53/SOX2 pathway in keratinocytes Journal of Investigative Dermatology 2018-09-01 [PMID: 29559340] (Bioassay, Mouse)
• E Reid, N Juleff, M Windsor, S Gubbins, L Roberts, S Morgan, G Meyers, E Perez-Mart, E Tchilian, B Charleston, J Seago Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of the Flaviviridae Suppress Cellular Immune Responses J Immunol, 2016-04-06;0(0):. 2016-04-06 [PMID: 27053760] (Bioassay, Mouse)
• g, Frentzen A, Gurlevik E, Yuan Q, Steinmann E, Ott M, Staeheli P, Schmid-Burgk J, Schmidt T, Hornung V, Kuehnel F, Pietschmann T Control of hepatitis C virus replication in mouse liver-derived cells by MAVS-dependent production of type I and type III interferons. J Virol, 2015-01-21;89(7):3833-45. 2015-01-21 [PMID: 25609814] (Bioassay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010-09-29;84(24):12703-12. 2010-09-29 [PMID: 20881038] (In Vivo, Mouse)
• Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE). PLoS ONE, 2010-01-14;5(1):e8720. 2010-01-14 [PMID: 20090936] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Ifnl3
• Uniprot:
  • NP_796370()