Recombinant Mouse IL-19 Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-19 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta . The ED50 for this effect is 20.0-200 pg/mL.
Source
E. coli-derived mouse IL-19 protein
Leu25-Ala176, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Il19
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2915-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-19 Protein, CF

  • IL-10C
  • IL19
  • IL-19
  • interleukin 19
  • MDA1
  • melanoma differentiation associated protein-like protein
  • NG.1Melanoma differentiation-associated protein-like protein
  • ZMDA1interleukin-19

Background

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24)(2 - 4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35 - 45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

  1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  3. Zdanov, (2004) Curr. Pharm. Des. 10:3873. 
  4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615. 
  5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
  6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
  7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
  8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
  13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.

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Publications for IL-19 (2915-IL/CF)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(4)
In Vivo
(3)
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Mouse
(7)
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Showing Publications 1 - 7 of 7.
Publications using 2915-IL/CF Applications Species
M Tsubaki, T Takeda, T Matsuda, Y Yamamoto, A Higashinak, K Yamamoto, K Tsurushima, T Ishizaka, S Nishida Interleukin 19 suppresses RANKL-induced osteoclastogenesis via the inhibition of NF-&amp;kappaB and p38MAPK activation and c-Fos expression in RAW264.7 cells Cytokine, 2021;144(0):155591. 2021 [PMID: 34052656] (Bioassay, Mouse) Bioassay Mouse
Manni M, Mandalapu S, McHugh K, Elloso M, Dudas P, Alcorn J Molecular Mechanisms of Airway Hyperresponsiveness in a Murine Model of Steroid-Resistant Airway Inflammation. J Immunol, 2016;196(3):963-77. 2016 [PMID: 26729801] (In Vivo, Mouse) In Vivo Mouse
Horiuchi H, Parajuli B, Wang Y, Azuma Y, Mizuno T, Takeuchi H, Suzumura A Interleukin-19 acts as a negative autocrine regulator of activated microglia. PLoS ONE, 2015;10(3):e0118640. 2015 [PMID: 25794104] (Bioassay, Mouse) Bioassay Mouse
Uto-Konomi A, Miyauchi K, Ozaki N Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines. PLoS ONE, 2012;7(7):e40343. 2012 [PMID: 22792286] (Bioassay, Mouse) Bioassay Mouse
Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012;109(25):9971-6. 2012 [PMID: 22665806] (Bioassay, Mouse) Bioassay Mouse
Hoffman C, Park SH, Daley E, Emson C, Louten J, Sisco M, de Waal Malefyt R, Grunig G Interleukin-19: a constituent of the regulome that controls antigen presenting cells in the lungs and airway responses to microbial products. PLoS ONE, 2011;6(11):e27629. 2011 [PMID: 22110701] (In Vivo, Mouse) In Vivo Mouse
Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010;84(24):12703-12. 2010 [PMID: 20881038] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol Il19
Uniprot