Recombinant Mouse Frizzled-1 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Frizzled-1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated Wnt-3a in a functional ELISA with an estimated K_D < 10 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Frizzled-1 protein
  

  Mouse Frizzled-1 (Gln72 - His248) with substitution Met122Ile Accession # O70421	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: O70421
• N-terminal Sequence: No results obtained: Gln 72 predicted & Ala74
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fzd1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 46 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Frizzled-1 Fc Chimera Protein, CF

• DKFZp564G072
• FLJ95923
• frizzled family receptor 1
• frizzled homolog 1
• frizzled, Drosophila, homolog of, 1
• Frizzled1
• Frizzled-1
• fz-1
• FZD1
• fzE1
• hFz1
• seven transmembrane spanning receptor
• Wnt receptor

Background

Frizzled-1 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1 - 4). Frizzled proteins are thought to be G-protein-coupled (1, 5). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta ‑catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzled-1 can also act as a Wnt antagonist, for example, when sequestered from LRP-5/6 by LRP-1 (6, 7). Mouse Frizzled-1 cDNA encodes 642 aa including a 68 aa signal peptide, an extracellular cysteine-rich domain (CRD, aa 106 - 225) that binds Wnts and is highly conserved among Frizzleds, a linker region, the 7TM region, and the C-terminal cytoplasmic domain (2, 3). Most Frizzleds, including Frizzled-1, contain a C‑terminal PDZ binding motif (1). Within aa 72 - 248, mouse Frizzled-1 shares 94%, 97%, 97% and 93% aa identity with human, rat, bovine and canine Frizzled‑1, respectively. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 8). Frizzled-1 shares high aa identity (~76%) with Frizzled-2 and -7, but may differ in expression and Wnt selectivity (4, 9). Specifically, Wnt-3a interaction with rat Frizzled-1, but not Frizzled-2, blocks toxicity of the Alzheimers A beta peptide on PC12 cells (9). In bone, Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 on mesenchymal cells (7, 10). Frizzled-1 is also one of the purported Wnt10b receptors whose signaling inhibits adipocyte differentiation (11). It is widely expressed, with mRNA detection in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (2, 3).

1. Schulte, G. and B. Vitezslav (2007) Trends Pharmacol. Sci. 28:518.
2. Xu, L.et al. (2001) Matrix Biol. 20:147.
3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
4. Gazit, A. et al. (1999) Oncogene 18:5959.
5. Liu, T. et al. (1999) J. Biol. Chem. 274:33539.
6. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
7. Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
8. Kaykas, A. et al. (2004) Nat. Cell Biol. 6:52.
9. Chacon, M.A. et al. (2008) J. Cell. Physiol. 217:215.
10. Yerges, L.M. et al. (2009) J. Bone Miner. Res. 24:87.
11. Bennett, C.N. et al. (2002) J. Biol. Chem. 277:30998.

Publications for Frizzled-1 (1120-FZ)(2)

Publications using 1120-FZ

• Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 2016-07-11;0(0):. 2016-07-11 [PMID: 27402827] (Bioassay)
• Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006-08-30;133(19):3787-96. 2006-08-30 [PMID: 16943279] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Fzd1
• Uniprot:
  • O70421()