Recombinant Mouse FGF-6 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse FGF-6 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.2‑1.0 ng/mL.
• Source: E. coli-derived mouse FGF-6 protein
  Pro39-Ile208
• Accession #: NP_034334
• N-terminal Sequence: Pro38
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fgf6
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF-6 Protein, CF

• FGF6
• FGF-6
• fibroblast growth factor 6
• HBGF-6
• Heparin secretory-transforming protein 2
• Heparin-binding growth factor 6
• HST2
• HST-2
• HSTF2
• HSTF-2

Background

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25 - 28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature mouse FGF-6 is a 171 aa protein that shares 94% and 99% aa sequence identity with human and rat FGF-6, respectively (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5 - 7). FGF-6 expression is restricted to skeletal muscle during development, although it can be upregulated in prostate cancer and Kaposi sarcoma (7 - 9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
3. de Lapeyriere, O. et al. (1990) Oncogene 5:823.
4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
6. Asada, M. et al. (1999) Growth Factors 16:293.
7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
8. de Lapeyriere, O. et al. (1993) Development 118:601.
9. Li, J. et al. (1993) Cancer 72:2253.
10. Floss, T. et al. (1997) Genes Dev. 11:2040.
11. Grass, S. et al. (1996) Development 122:141.

Publications for FGF-6 (5750-F6/CF)(1)

Publication using 5750-F6/CF

• S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 2019-06-11;9(1):8470. 2019-06-11 [PMID: 31186471] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Fgf6
• Uniprot:
  • NP_034334(Mouse)