Recombinant Mouse FCRLB/FCRY Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse FCRLB/FCRY Protein, CF Summary

• Details of Functionality: Measured by its ability to bind mouse IgG with an estimated K_D < 300 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse FCRLB/FCRY protein
  Ala18-Ser427, with a C-terminal 6-His tag
• Accession #: Q5DRQ8
• N-terminal Sequence: Ala18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fcrlb
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FCRLB/FCRY Protein, CF

• Fc receptor homolog expressed in B cells protein 2
• Fc receptor like 2
• Fc receptor-like and mucin-like 2
• Fc receptor-like and mucin-like protein 2
• Fc receptor-like B
• Fc receptor-like protein 2
• Fc receptor-related protein Y
• FcRL2
• FCRL2FCRLM2
• FCRLB
• FCRLM2
• FCRLY
• FCRY
• FLJ31052
• FREB2
• FREB-2
• FREB-2FcRY
• RP11-474I16.6

Background

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82% and 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1 - 3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is up‑regulated in B cells by BAFF and LPS (3). Among non‑hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in non‑proliferating cells and at the onset of apoptosis (3, 5).

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
3. Masuda, K. et al. (2005) Gene 363:32.
4. Chikaev, N.A. et al. (2005) Genomics 85:264.
5. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
6. Inozume, T. et al. (2005) Int. J. Cancer 114:283.

Bioinformatics

• Gene Symbol: Fcrlb
• Uniprot:
  • Q5DRQ8()