Recombinant Mouse EphA5 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse EphA5 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmEphA5 at 2 µg/mL (100 µL/well) can bind rmEphrin-A2/Fc Chimera with a linear range of 0.16-10 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse EphA5 protein
  Ser58-Pro412, with a C-terminal 6-His tag
• Accession #: Q60629
• N-terminal Sequence: Ser58
• Protein/Peptide Type: Recombinant Proteins
• Gene: Epha5
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 40.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-50 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EphA5 Protein, CF

• Bsk
• Cek7
• EC 2.7.10
• EC 2.7.10.1
• Ehk1
• EHK-1
• EHK1Hek7
• EK7
• EPH homology kinase 1
• Eph homology kinase-1
• EPH receptor A5
• EphA5
• EPH-like kinase 7
• ephrin type-A receptor 5
• Hek7
• receptor protein-tyrosine kinase HEK7
• Rek7
• TYRO4HEK7CEK7
• tyrosine-protein kinase receptor EHK-1

Background

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1 - 4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The mouse EphA5 cDNA encodes an 877 amino acid (aa) precursor that includes a 26 aa signal sequence, a 386 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 444 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Taking into account differences in splice variants between species, comparable regions of the mouse EphA5 ECD share greater than 97% aa sequence identity with bovine, human, and rat EphA5, and 27 - 43% aa identity with mouse EphA1, 2, 3, 4, 6, 7, and 8. Multiple EphA5 splice variants exist and differ in their extracellular and intracellular sequences (5). Mouse EphA5 is expressed exclusively in brain with the highest levels in the hippocampus and limbic system (5). During embryonal development, EphA5 is preferentially expressed in neurons migrating away from the ventricular zone (5). The interaction of EphA5 with its ligands is also involved in the topographic projection of hippocampal, olfactory, and retinotectal neurons (6). Interference in this system by the transgenic expression of soluble EphA5 implicates hippocampal post-synaptic areas for native EphA5 function (7).

1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
5. Zhou, R. et al. (1994) J. Neurosci. Res. 37:129.
6. Zhou, R. (1997) Cell Tissue Res. 290:251.
7. Martinez, A. et al. (2005) Neuroscience 135:451.

Publications for EphA5 (3037-A5)(3)

Publications using 3037-A5

• O Ros, S Baudet, Y Zagar, K Loulier, F Roche, S Couvet, A Aghaie, M Atkins, A Louail, C Petit, C Metin, Y Mechulam, X Nicol SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation Cell Rep, 2020;32(3):107934. 2020 [PMID: 32697983] (Cell Culture, Mouse)
• JW Hughes, JH Cho, HE Conway, MR DiGruccio, XW Ng, HF Roseman, D Abreu, F Urano, DW Piston Primary cilia control glucose homeostasis via islet paracrine interactions Proc. Natl. Acad. Sci. U.S.A., 2020;117(16):8912-8923. 2020 [PMID: 32253320] (Bioassay, Mouse)
• Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets. Cell, 2007;129(2):359-70. 2007 [PMID: 17448994] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Epha5
• Uniprot:
  • Q60629()