Recombinant Mouse ECM1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ECM1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells.

  When 2.5 x 10⁴ cells/well are added to rmECM-1 coated plates (5 µg/mL, 100 µL/well), approximately 55‑85% will adhere after 60 minutes at 37° C.

  Optimal dilutions should be determined by each laboratory for each application.

• Source: Mouse myeloma cell line, NS0-derived mouse ECM1 protein
  Ala20-Glu559, with a C-terminal 6-His tag
• Accession #: AAI38694
• N-terminal Sequence: Ala20
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ecm1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 61.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ECM1 Protein, CF

• ECM1
• extracellular matrix protein 1
• p85
• Secretory Component Glycoprotein
• Secretory Component P85

Background

Extracellular matrix protein-1 (ECM-1, ECM-1a) is an 85 kDa, secreted glycoprotein important in connective tissue organization (1 - 3). Of identified splice variants, the 559 amino acid (aa) form, ECM-1a is most widely expressed, with highest expression in the placenta, heart, and developing bones (3, 4). ECM-1b (434 aa) is found only in tonsil and skin, where it is associated with suprabasal keratinocytes (3, 5). Mouse ECM-1 contains a 19 aa signal peptide and a 540 aa secreted portion that includes an N-terminal proline-rich, cysteine-free region, two tandem repeat domains, and a C-terminal domain. Mature mouse ECM-1 shares 90% aa identity with rat ECM-1 and 65 - 69% aa identity with corresponding isoforms of human, equine, bovine and canine ECM-1. There are six repeats of a CC(X_{7 - 10})C motif (x = any aa) within the tandem repeat and C-terminal domains. These motifs, also found in members of the albumin family, are expected to form two (in ECM-1b) or three (in ECM-1a) “double loop” structures that are involved in ligand binding to extracellular matrix molecules such as fibulin-1, perlecan, laminin 332, and fibronectin (4 - 7). ECM-1 is over-expressed in many malignant epithelial tumors and has demonstrated angiogenic activity (8, 9). A role in regulating alkaline phosphatase during endochondral bone formation has also been suggested (4). In humans, loss of function within the tandem repeat regions due to mutation is considered causative of thickened and irregular extracellular matrix within connective tissue, called lipoid proteinosis (10). Autoantibodies in the skin disease lichen sclerosis also target these repeats (11). The phenotypes of these diseases support a role for ECM-1 as a “biological glue” in the dermis (1, 6, 7).

1. Chan, I. (2004) Exp. Dermatol. 29:52.
2. Bhalerao, J. et al. (1995) J. Biol. Chem 270:16385.
3. Smits, P. et al. (1997) Genomics 45:487.
4. Deckers, M.M.L. et al. (2001) Bone 28:14.
5. Smits, P. et al. (2000) J. Invest. Dermatol. 114:718.
6. Fujimoto, N. et al. (2005) Biochem. Biophys. Res. Commun. 333:1327.
7. Sercu, S. et al. (2008) J. Invest. Dermatol. 128:1397.
8. Han, Z. et al. (2001) FASEB J. 15:988.
9. Wang, L. et al. (2003) Cancer Lett. 200:57.
10. Hamada, T. et al. (2003) J. Invest. Dermatol. 120:345.
11. Oyama, N. et al. (2004) J. Clin. Invest. 113:1550.

Bioinformatics

• Gene Symbol: Ecm1
• Uniprot:
  • AAI38694()