Recombinant Mouse DLL1 Fc Chimera Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse DLL1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.25-1 μg/mL.
Mouse myeloma cell line, NS0-derived mouse DLL1 protein
Mouse DLL1
Accession # Q61483
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
81.4 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
94 kDa, reducing conditions
Read Publications using
5026-DL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse DLL1 Fc Chimera Protein, CF

  • delta (Drosophila)-like 1
  • Delta 1
  • Delta
  • Delta1
  • delta-like 1 (Drosophila)
  • delta-like protein 1
  • DL1
  • DLL1
  • Drosophila Delta homolog 1
  • H-Delta-1


Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature mouse DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, mouse DLL1 shares 91% and 95% aa sequence identity with human and rat DLL1, respectively. It shares 26%, 35%, and 51% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment, released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (6-12). The up-regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (13). DLL1 is also over-expressed in cervical carcinoma and glioma and contributes to tumor progression (14, 15).

  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  6. Takahashi, Y. et al. (2003) Development 130:4259.
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Shimojo, H. et al. (2008) Neuron 58:52.
  12. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537.
  13. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  14. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  15. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.

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Publications for DLL1 (5026-DL)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 2 applications: Bioassay, Cell Culture.

Filter By Application
Cell Culture
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 5026-DL Applications Species
S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32820046] (Bioassay, Mouse) Bioassay Mouse
E Silkensted, F Arenas, B Colom-Sanm, S Xargay-Tor, M Higashi, A Giró, V Rodriguez, P Fuentes, WE Aulitzky, H van der Ku, S Beà, ML Toribio, E Campo, M López-Guer, D Colomer Notch1 signaling in NOTCH1-mutated mantle cell lymphoma depends on Delta-Like ligand 4 and is a potential target for specific antibody therapy J. Exp. Clin. Cancer Res., 2019;38(1):446. 2019 [PMID: 31676012] (Cell Culture, Mouse) Cell Culture Mouse
KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling Elife, 2018;7(0):. 2018 [PMID: 30589410] (Bioassay, Mouse) Bioassay Mouse
L Liu, GW Charville, TH Cheung, B Yoo, PJ Santos, M Schroeder, TA Rando Impaired Notch Signaling Leads to a Decrease in p53 Activity and Mitotic Catastrophe in Aged Muscle Stem Cells Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30244867] (Cell Culture, Mouse) Cell Culture Mouse
H Takeuchi, M Schneider, DB Williamson, A Ito, M Takeuchi, PA Handford, RS Haltiwange Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30127001] (Bioassay, Mouse) Bioassay Mouse
Varshney S, Stanley P Notch Ligand Binding Assay Using Flow Cytometry. Bio Protoc, 0;7(23):. 0 [PMID: 29333477] (Bioassay, Mouse) Bioassay Mouse
S Varshney, P Stanley Notch Ligand Binding Assay Using Flow Cytometry Bio Protoc, 2017;7(23):. 2017 [PMID: 29333477] (Bioassay, Human) Bioassay Human
T Kostrzewsk, AJ Borg, Y Meng, I Filipovic, V Male, A Wack, PA DiMaggio, HJM Brady Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development J. Immunol., 2018;0(0):. 2018 [PMID: 29311361] (Bioassay, Mouse) Bioassay Mouse
Y Zhang, G Zhu, H Xiao, X Liu, G Han, G Chen, C Hou, B Shen, Y Li, N Ma, R Wang CD19 regulates ADAM28-mediated Notch2 cleavage to control the differentiation of marginal zone precursors to MZ B cells J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28707394] (Bioassay, Mouse) Bioassay Mouse
D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren Selective regulation of Notch ligands during angiogenesis is mediated by vimentin Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28533359] (Bioassay, Mouse) Bioassay Mouse
Show All 12 Publications.

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Gene Symbol Dll1