Recombinant Mouse DLL1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse DLL1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.25-1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse DLL1 protein
Mouse DLL1
(Ser22-Gln516)
Accession # Q61483
LIEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Dll1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
81.4 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
94 kDa, reducing conditions
Publications
Read Publications using
5026-DL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse DLL1 Fc Chimera Protein, CF

  • delta (Drosophila)-like 1
  • Delta 1
  • Delta
  • Delta1
  • delta-like 1 (Drosophila)
  • delta-like protein 1
  • DL1
  • DLL1
  • Drosophila Delta homolog 1
  • H-Delta-1

Background

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature mouse DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, mouse DLL1 shares 91% and 95% aa sequence identity with human and rat DLL1, respectively. It shares 26%, 35%, and 51% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment, released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (6-12). The up-regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (13). DLL1 is also over-expressed in cervical carcinoma and glioma and contributes to tumor progression (14, 15).

  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  6. Takahashi, Y. et al. (2003) Development 130:4259.
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Shimojo, H. et al. (2008) Neuron 58:52.
  12. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537.
  13. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  14. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  15. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.

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5026-DL
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Applications: Bioactivity

Publications for DLL1 (5026-DL)(7)

We have publications tested in 2 confirmed species: Mouse, Hamster.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(7)
All Applications
Filter By Species
Mouse
(6)
Hamster
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using 5026-DL Applications Species
H Takeuchi, M Schneider, DB Williamson, A Ito, M Takeuchi, PA Handford, RS Haltiwange Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30127001] (Bioassay, Mouse) Bioassay Mouse
Varshney S, Stanley P Notch Ligand Binding Assay Using Flow Cytometry. Bio Protoc, 0;7(23):. 0 [PMID: 29333477] (Bioassay, Mouse) Bioassay Mouse
T Kostrzewsk, AJ Borg, Y Meng, I Filipovic, V Male, A Wack, PA DiMaggio, HJM Brady Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development J. Immunol., 2018;0(0):. 2018 [PMID: 29311361] (Bioassay, Mouse) Bioassay Mouse
Y Zhang, G Zhu, H Xiao, X Liu, G Han, G Chen, C Hou, B Shen, Y Li, N Ma, R Wang CD19 regulates ADAM28-mediated Notch2 cleavage to control the differentiation of marginal zone precursors to MZ B cells J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28707394] (Bioassay, Mouse) Bioassay Mouse
D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren Selective regulation of Notch ligands during angiogenesis is mediated by vimentin Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28533359] (Bioassay, Mouse) Bioassay Mouse
LeBon, Lauren, Lee, Tom V, Sprinzak, David, Jafar-Nejad, Hamed, Elowitz, Michael Fringe proteins modulate Notch-ligand cis and trans interactions to specify signaling states. Elife, 2014;3(0):e02950. 2014 [PMID: 25255098] (Bioassay, Hamster) Bioassay Hamster
Driver E, Sillers L, Coate T, Rose M, Kelley M The Atoh1-lineage gives rise to hair cells and supporting cells within the mammalian cochlea. Dev Biol, 2013;376(1):86-98. 2013 [PMID: 23318633] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Dll1
Uniprot