Recombinant Mouse Dkk-4 Protein, CF

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Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-4 (Catalog # 3105‑DK/CF) inhibits a constant ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Mouse Dkk-4 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1-4 µg/mL.
Optimal concentrations should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse Dkk-4 protein
Leu19-Ile221 (Arg67Ser and Arg70Ser), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu19
Protein/Peptide Type
Recombinant Proteins
Gene
Dkk4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
31 kDa, reducing conditions
Publications
Read Publication using
3105-DK/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Dkk-4 Protein, CF

  • dickkopf (Xenopus laevis) homolog 4
  • dickkopf homolog 4 (Xenopus laevis)
  • dickkopf-4
  • dickkopf-related protein 4
  • Dkk4
  • Dkk-4
  • hDkk-4
  • MGC129562
  • MGC129563

Background

Dickkopf related protein 4 (Dkk-4) is a member of the Dickkopf protein family that includes Dkk-1, -2, -3, and -4 and a related protein, Soggy (1). Expression of Dkk-4 has only been shown with sensitive PCR techniques during early embryonic development in mice (2), in differentiated human ES cells (3), or in mice that express dominant-active beta -catenin elevating Wnt signaling in the forebrain (4). Dkk proteins are secreted proteins that are synthesized as precursors with an N-terminal signal peptide; all have two conserved cysteine-rich domains separated by a linker region which contains a potential furin type proteolytic cleavage site. The domains contain 10 cysteines each; prokineticin and colipase families show a configuration of cysteines similar to the second motif (5). Mouse Dkk-4 shows 91%, 72% and 71% amino acid (aa) identity with rat, human and canine Dkk-4, respectively, and 40 - 46% aa identity with other mouse Dkk proteins. Dkk-4 is predicted as a 25 kDa protein, but transfection of 293T cells produces a shorter (15 - 17 kDa) form containing only the second cysteine-rich domain, as well as longer (30 - 32 and 40 kDa) forms that do not appear to be glycosylated or form covalent multimers (1). Of the four Dkk proteins, Dkk-4 is the most like Dkk-1. Both are unequivocal antagonists of the canonical Wnt signaling pathway (1, 6), which is activated by Wnt protein engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (7). Dkk-1 and Dkk-4 antagonize Wnt by direct high-affinity binding to LRP5/6, forming ternary complexes of LRP5/6 with the Kremens protein Krm2. Internalization of the complex is triggered, making LRP5/6 unavailable for interaction with Wnt ligands (6 - 9).

  1. Krupnik, V.E. et al. (1999) Gene 238:301. 
  2. Kemp, C. et al. (2005) Dev. Dyn. 233:1064. 
  3. Katoh, Y. and M. Katoh (2005) Int. J. Mol. Med. 16:477. 
  4. Diep, D.B. et al. (2004) Dev. Brain Res. 153:261. 
  5. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
  6. Mao, B. and C. Niehrs (2003) Gene 302:179.
  7. Logan, C.Y. and R. Nusse (2004) Ann. Rev. Cell Dev. Biol. 20:781.
  8. Mao, B. et al. (2002) Nature 417:664.
  9. Mao, B. et al. (2001) Nature 411:321.

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Publications for Dkk-4 (3105-DK/CF)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Dkk4
Entrez
Uniprot