Recombinant Mouse CRISP-1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CRISP-1 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit proliferation/survival of the 3A‑sub E human placenta cell line. The ED₅₀ for this effect is 0.12-0.6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CRISP-1 protein
  Gln20-His244, with a C-terminal 6-His tag
• Accession #: Q03401
• N-terminal Sequence: Gln20 predicted: No results obtained, sequencing might be blocked.
• Protein/Peptide Type: Recombinant Proteins
• Gene: Crisp1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30 kDa, reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CRISP-1 Protein, CF

• Acidic epididymal glycoprotein homolog
• acidic epididymal glycoprotein-like 1
• AEGL1
• AEG-related protein
• ARPAEG-like protein
• CRISP1
• CRISP-1
• CRISP-1AEGL1HSCRISP1D
• cysteine-rich secretory protein 1
• cysteine-rich secretory protein-1 delta
• HSCRISP1G
• HUMARP

Background

Cysteine‑rich secretory protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 32 kDa protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N‑terminal SCP/CAP domain, a hinge region, and a Cys‑rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP‑1 in mouse and rat is CRISP‑4 (5, 6). Mature mouse CRISP‑1 shares 68% amino acid (aa) sequence identity with rat CRISP‑1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP‑2, ‑3, and ‑4, respectively. Rodent CRISP‑1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7 ‑ 9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP‑1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP‑1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13 ‑ 15).

1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
3. Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
4. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
7. Charest, N.J. et al. (1989) Endocrinology 125:942.
8. Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
9. Nixon, B. et al. (2006) Biol. Reprod. 74:275.
10. Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
11. Busso, D. et al. (2007) Biol. Reprod. 77:848.
12. Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
13. Haendler, B. et al. (1993) Endocrinology 133:192.
14. Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
15. Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.

Bioinformatics

• Gene Symbol: Crisp1
• Uniprot:
  • Q03401()