Recombinant Mouse Contactin-3 Protein, CF

When Recombinant Mouse Contactin-3 (Catalog # 9800‑CN) iscoated onto a microplate at 2 µg/mL, Recombinant Human Amyloid PrecursorProtein (APP) Chimera binds with an ED50 of 0.02-0.1 μg/mL

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Contactin-3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Contactin‑3 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Amyloid precursor protein (APP) Fc Chimera that produces 50% optimal binding response is 0.02-0.1 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Contactin-3 protein
  Met1-Thr1003, with a C-terminal 10-His tag
• Accession #: AAA17403
• N-terminal Sequence: Glu20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-157 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Contactin-3 Protein, CF

• BIG-1
• CNTN3
• contactin 3 (plasmacytoma associated)
• Contactin3
• Contactin-3
• PANG
• PCS

Background

Contactin-3 (CNTN-3), also known as brain-derived immunoglobulin superfamily protein 1 (BIG-1) and plasmacytoma-associated neuronal glycoprotein (PANG), is an axon-associated cell adhesion molecule (AxCAM). CNTN-3, together with 5 additional contactin proteins, forms the TAG-1/F3 subgroup of the Ig superfamily (1-3). Mature mouse CNTN-3 is a heavily glycosylated, 983 amino acid (aa) chain containing 6 Ig C2-like domains, 4 type III fibronectin-like domains, and a GPI anchor (1, 2). Mouse CNTN-3 shares 92% and 95% aa identity with human and rat CNTN-3, respectively. CNTN-3 is abundantly expressed in the adult brain, particularly in the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (1, 4). In mice, CNTN-3 has been associated with sensory circuitry such as the olfactory bulb and the outer segment of the retina (5, 6). Immobilized CNTN-3 promotes neurite outgrowth from rat hippocampal neurons (4). Although the physiological function of CNTN-3 is still poorly understood, experimental evidence shows that it forms both cis- and trans­-complexes with protein tyrosine phosphatase gamma (PTPRG) at the cell surface (5, 6).

1. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
2. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
3. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
4. Kamei, Y. et al. (2000) Genomics 69:113.
5. Bouyain, S. and Watkins, D. (2009) PNAS 107:2443.
6. Nikolaienko, R. et al. (2016) J. Biol. Chem. 291:21335.

Bioinformatics

• Uniprot:
  • AAA17403()