Recombinant Mouse CD300c2 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD300c2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and serially diluted Recombinant Mouse CD300c2 Fc Chimera.
  The ED₅₀ for this effect is 0.6‑3 µg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse protein
  

  Mouse CD300c2 (Cys22-Ser193) Accession # BAC80269	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: BAC80269
• N-terminal Sequence: Cys22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd300c
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD300c2 Fc Chimera Protein, CF

• Cd300c;Cd300d;CLM4;CLM-4;CMRF-35-like molecule 4;DIg;DIgR1;Igs;Igsf7;LM;Lmir2;MA;MAIR-II
• CD300c2
• CLM4
• IGSF7
• LMIR2
• MAIR-II

Background

Leukocyte mono-Ig-like receptor 2 (LMIR2), also known as CMRF-35A, CD300c2, CLM4, DIgR1, and MAIR-II, is a 35 kDa type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules (1). Mature mouse LMIR2 consists of a 165 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 20 aa cytoplasmic tail (2). An alternate splice form lacks two amino acids C-terminal to the Ig-like domain (3). Within the ECD, mouse LMIR2 shares 45% aa sequence identity with human LMIR2. The CMRF-35 antibody detects both LMIR2 and LMIR1/CMRF-35H which are 92% identical in their Ig-like domains (4-7). LMIR2 is expressed on the surface of subpopulations of bone marrow derived dendritic cells and mast cells, monocytes, peritoneal macrophages, marginal zone B cells, and T cells (2, 3, 6, 8). It contains at least one positively charged amino acid in its transmembrane segment which may mediate LMIR2 association with the adaptor signaling proteins DAP10, DAP12, and FcR gamma (3, 8). Cross-linking of LMIR2 on macrophages induces the secretion of the proinflammatory molecules TNF-alpha , IL-6, and CCL2 (3).

1. Clark, G.J. et al. (2001) Tissue Antigens 57:415.
2. Luo, K. et al. (2001) Biochem. Biophys. Res. Commun. 287:35.
3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
4. Jackson, D.G. et al. (1992) Eur. J. Immunol. 22:1157.
5. Daish, A. et al. (1993) Immunology 79:55.  
6. Green, B.J. et al. (1998) Int. Immunol. 10:891.
7. Clark, G.J. et al. (2007) J. Leukoc. Biol. 82:1126.
8. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.

Bioinformatics

• Gene Symbol: Cd300c
• Uniprot:
  • BAC80269()