Recombinant Mouse CD160 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD160 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA.

  When Recombinant Mouse HVEM Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD160 that produces 50% of the optimal binding response is found to be approximately 0.012 ‑ 0.06 μg/mL.

• Source: Chinese Hamster Ovary cell line, CHO-derived mouse CD160 protein
  Gly28-Ser160 with a C-terminal 6-His tag
• Accession #: AAH21596
• N-terminal Sequence: Gly28 & Ile30
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd160
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25-40 kDa, reducing conditons

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD160 Protein, CF

• BY55
• BY55FLJ46513
• CD160 antigenimmunoglobulin superfamily member
• CD160 molecule
• CD160
• Natural killer cell receptor BY55
• NK1
• NK28

Background

CD160 (also Natural killer cell receptor BY55) is a 16 kDa (predicted) member of the Ig superfamily (1 ‑ 4). In mouse, it is expressed principally on nonmyeloid hematopoietic cells. These include CD3+ NK1.1 cells, CD8+ T_EM and T_CM T cells, CD8 alpha + IELs, NKT cells, CD8-gamma δ TCR T cells, and vascular endothelial cells (1, 5 - 7). Mouse CD160 has been identified as a 20 - 21 kDa GPI-linked glycoprotein (4, 5). It is synthesized as a preproprotein that is 185 amino acids (aa) in length. The precursor contains a 27 aa signal sequence, a 133 aa mature molecule that shows one 98 aa V-type Ig-like domain (aa 28 - 125), and a 25 aa prosegment that is cleaved to generate a GPI-linkage at Ser160. Mouse GPI-linked CD160 is known to be cleaved by phospholipase C, and this generates a 40 kDa (presumably dimeric) band in SDS-PAGE (5). One alternative splice form for mouse CD160 is reported that appears to show a deletion of aa 137 - 180. This may generate a soluble molecule (5; GenBank Accession # NP_001156969). Mature mouse CD160 shares 63% and 88% aa identity with human and rat CD160, respectively.

In mouse, CD160 is reported to bind to bind to HVEM/TNFRSF14, and both classical and non‑classical MHC Class I molecules (5, 8). MHC‑I proteins recognized by CD160 include Dd, Kb, Qa-1b and CD1d (5). Upon engagement, the effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to human HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on NK1.1 cells, mouse CD160 ligation alone has no effect; when combined with NK1.1 antigen stimulation, CD160 decreases NK cell IFN-gamma secretion. Relative to cytotoxicity, NK cell activity is positively correlated with the presence of CD160 (5).

1. Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244.
2. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
3. Maiza, H. et al. (1993) J. Exp. Med. 178:1121.
4. Anumanthan, A. et al. (1998) J. Immunol. 161:2780.
5. Maeda, M. et al. (2005) J. Immunol. 175:4426.
6. Fons, P. et al. (2006) Blood 108:2608.
7. Tsujimura, K. et al. (2006) Immunol. Lett. 48:106.
8. Cheung, T.C. et al. (2009) Proc. Natl. Acad. Sci USA 106:6244.

 

Bioinformatics

• Gene Symbol: Cd160
• Uniprot:
  • AAH21596()