Recombinant Mouse APCDD1 Protein, CF

Recombinant Mouse APCDD1 (Catalog # 9981-AP) binds to Biotinylated Recombinant MouseWnt-3a (Catalog # BT1324) with an ED50 of 1‑8 µg/mL.

2 μg/lane of Recombinant Mouse APCDD1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-65 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse APCDD1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Mouse APCDD1 binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) . The ED₅₀ for the binding is 1-8 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse APCDD1 protein
  Ser25-Thr492, with a C-terminal 6-His tag
• Accession #: Q3U128
• N-terminal Sequence: Ser25 & Leu27
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse APCDD1 Protein, CF

• Adenomatosis polyposis coli down-regulated 1 protein
• adenomatosis polyposis coli down-regulated 1
• APC Down Regulated 1
• APCDD1
• B7323
• DRAPC1
• HYPT1
• protein APCDD1

Background

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature mouse APCDD1 consists of a 466 amino acid (aa) extracellular domain (ECD) , a 21 aa transmembrane domain, and a 1 aa cytoplasmic region. Mouse APCDD1 shares a 98% and 94% amino acid sequence similarity with rat and human respectively. APCDD1 interacts in vitro with Wnt3A and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-precipitate with recombinant Wnt3A and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt3a and LRP5 at the cell surface.

1. Mazzoni, J. et al. (2017) Neuron. 96:1055.
2. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
3. Shimomura, Y. (2010) Nature 464:1043.
4. Kandimalla, R. (2017) Oncogenesis 6:e308.
5. Yutaka Shimomura, et al. (2010) Nature 464:1043.
6. Sung-Gook CHO, (2017) Oncology Letters 14:4845.
7. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.

Bioinformatics

• Uniprot:
  • Q3U128()