Recombinant Human XCL2/SCM-1 beta Protein

Recombinant Human XCL2/SCM-1 beta (Catalog # 8418-X2) chemoattracts BaF3 mouse pro B cells transfected with human XCR1. The ED50 for this effect is 10-60 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human XCL2/SCM-1 beta Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human XCR1. The ED₅₀ for this effecy is 10-60 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human XCL2/SCM-1 beta protein
  Val22-Gly114
• Accession #: Q9UBD3
• N-terminal Sequence: Val22
• Protein/Peptide Type: Recombinant Proteins
• Gene: XCL2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19-22 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human XCL2/SCM-1 beta Protein

• C motif chemokine 2
• chemokine (C motif) ligand 2
• cytokine SCM-1 beta
• SCM1 beta
• SCM-1 beta
• SCM1B
• SCM-1b
• SCYC2
• SCYC2XC chemokine ligand 2
• small inducible cytokine subfamily C, member 2
• XCL2

Background

Human XCL2 (also known as Lymphotactin-2, SCYC2, and SCM-1 beta ) is a 10 kDa glycosylated protein in the C chemokine family (1-3). XCL1/Lymphotactin is a closely related chemokine that differs from XCL2 by only two amino acids (aa). Human XCL2 encodes a 114 amino acid aa precursor protein with a 21 aa predicted signal peptide. Members of the C chemokine family lack two (the 1^st and 3^rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines. They also have an extended carboxy terminus (4). XCL1/Lymphotactin which is expressed in activated T cells and NK cells, while certain stimulatory conditions have been shown to induce XCL2 expression in macrophages (5-7). XCL2 and XCL1/Lymphotactin bind and activate the G protein-coupled receptor, XCR1. XCR1 is expressed on dendritic cells, T lymphocytes, and B lymphocytes (8-11). Binding of XCL2 to XCR1 initiates chemotaxic cell migration in dendritic cells as well as in T and B lymphocytes. XCR1 is also expressed on ovarian carcinoma cells and may contribute to cancer cell migration and proliferation in response to XCL2 or XCL1/Lymphotactin (12).

1. Yoshida, T. et al. (1996) FEBS Lett. 395:82.
2. Kelner, G.S. et al. (1994) Science 266:1395.
3. Muller, S. et al. (1995) Eur. J. Immunol. 25:1744.
4. Yoshida, T. et al. (1995) FEBS Lett. 360:155.
5. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
6. Luzi, G. et al. (2007) Clin. Immunol. 125:127.
7. Cao, W. et al. (2008) Curr. Microbiol. 57:189.
8. Kroczek, R.A. and V. Henn (2012) Front. Immunol. 3:14.
9. Huang, H. et al. (2001) Biochem. Biophys. Res. Commun. 281:378.
10. Pigard, N. et al. (2009) J. Neuroimmunol. 209:114.
11. Yamazaki, C. et al. (2010) Biochem. Biophys. Res. Commun. 397:756.
12. Kim, M. et al. (2012) Mol. Cancer. Res. 10:1419.

Bioinformatics

• Gene Symbol: XCL2
• Uniprot:
  • Q9UBD3()