Recombinant Human Wnt-5b Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human Wnt-5b Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 125-750 ng/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-5b protein
Gln18-Lys359
Accession #
N-terminal Sequence
Gln18 predicted: No results obtained, sequencing might be blocked
Protein/Peptide Type
Recombinant Proteins
Gene
WNT5B
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
38.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45 kDa, reducing conditions
Publications
Read Publications using
7347-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-5b Protein, CF

  • MGC2648
  • protein Wnt-5b
  • wingless-type MMTV integration site family, member 5B
  • WNT-5B protein
  • Wnt5b
  • Wnt-5b

Background

Wnt proteins are cysteine‑rich secreted glycoproteins that play critical roles in both carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low‑density lipoprotein receptor‑related protein family (LRP‑5 or ‑6), or the Ryk atypical receptor tyrosine kinase (1‑3). Downstream effects of Wnt signaling occur through multiple pathways with differing intracellular components: the canonical Wnt/ beta ‑catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (PCP) pathway (1‑4). Wnt‑5b is a 49 kDa glycoprotein that is implicated in the Wnt/Ca2+ and PCP pathways (4‑8). These pathways can inhibit canonical Wnt/ beta ‑catenin signaling (4, 7). Human and mouse Wnt‑5b are synthesized as 359 amino acid (aa) precursors that contain a 17 aa signal sequence and a 342 aa mature region. Mature human Wnt‑5b shares 95%, 94%  90%, 89% and 86% aa identity with mature mouse, rat, bovine, chick and Xenopus Wnt‑5b, respectively. Although Wnt‑5a and Wnt‑5b share 83% aa identity, their effects may be different. For example, Wnt-5b, but not Wnt‑5a, promotes cell cycle progression and is weakly transforming (8, 9). Wnt‑5a and ‑5b are thought to coordinate developmental events, such as chondrocyte differentiation and formation of endochondral bone (5, 6, 10). In contrast to more focused expression of Wnt‑5a, Wnt‑5b is constitutively expressed at low levels throughout mouse embryonic development (10, 11). In adult mice, Wnt-5b is widely expressed, including heart, liver, brain, lung, testes, kidney, and pancreas (11‑13). Wnt‑5b is up‑regulated during early adipogenesis, and its overexpression in 3T3‑L1 cells partially inhibits canonical Wnt suppression of adipogenesis (7, 14). It enhances PPAR gamma expression and promotes differentiation of preadipocytes (14). Human Wnt‑5b polymorphisms have been associated with Type II diabetes (12).

  1. Mikels, A.J. & R. Nusse, 2006, Oncogene 25:7461.
  2. Miller, J.R. (2002) Genome Biol. 3:3001.
  3. Li, L et al. (2004) J. Neurosci 29:5873.
  4. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  5. Fazzi, R. et al. (2011) PLoS ONE 6:e25600.
  6. Bradley, E.W. and M.H. Drissi (2011) J. Cell. Physiol. 226:1683.
  7. Kanazawa, A. et al. (2005) Biochem. Biophys. Res. Com. 330:505.
  8. Wong, G.T. et al. (1994) Mol. Cell. Biol. 14:6278.
  9. Yang, Y. et al. (2003) Development 130:1003.
  10. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  11. Gavin, B. J. et al. (1990) Genes Dev. 4:2319.
  12. Kanazawa, A. et al. (2004) Am. J. Hum. Genet. 75:832.
  13. Heller, R.S. et al. (2002) Dev. Dyn. 225:260.
  14. van Tienen, F.H.J. et al. (2009) Biochem. Biophys. Res. Commun. 387:207.

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Publications for Wnt-5b (7347-WN/CF)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, ELISA Standard.


Filter By Application
Bioassay
(6)
ELISA Standard
(1)
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Human
(7)
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Showing Publications 1 - 7 of 7.
Publications using 7347-WN/CF Applications Species
Tumour cell-derived WNT5B modulates in vitro lymphangiogenesis via induction of partial endothelial-mesenchymal transition of lymphatic endothelial cells Oncogene, 2016;0(0):. 2016 [PMID: 27593938] (ELISA Standard, Human) ELISA Standard Human
Angbohang A, Wu N, Charalambous T, Eastlake K, Lei Y, Kim Y, Sun X, Limb G Downregulation of the Canonical WNT Signaling Pathway by TGFbeta1 Inhibits Photoreceptor Differentiation of Adult Human Muller Glia with Stem Cell Characteristics. Stem Cells Dev, 2015;25(1):1-12. 2015 [PMID: 26456050] (Bioassay, Human) Bioassay Human
Choudhary P, Dodsworth B, Sidders B, Gutteridge A, Michaelides C, Duckworth J, Whiting P, Benn C A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells. PLoS ONE, 2015;10(6):e0130379. 2015 [PMID: 26121260] (Bioassay, Human) Bioassay Human
Nicenboim, J, Malkinson, G, Lupo, T, Asaf, L, Sela, Y, Mayseless, O, Gibbs-Bar, L, Senderovich, N, Hashimshony, T, Shin, M, Jerafi-Vider, A, Avraham-Davidi, I, Krupalnik, V, Hofi, R, Almog, G, Astin, J W, Golani, O, Ben-Dor, S, Crosier, P S, Herzog, W, Lawson, N D, Hanna, J H, Yanai, I, Yaniv, K Lymphatic vessels arise from specialized angioblasts within a venous niche. Nature, 2015;0(0):. 2015 [PMID: 25992545] (Bioassay, Human) Bioassay Human
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human) Bioassay Human
Keats E, Dominguez J, Grant M, Khan Z Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells, 2014;32(6):1649-60. 2014 [PMID: 24496952] (Bioassay, Human) Bioassay Human
Takeshita A, Iwai S, Morita Y, Niki-Yonekawa A, Hamada M, Yura Y Wnt5b promotes the cell motility essential for metastasis of oral squamous cell carcinoma through active Cdc42 and RhoA. Int J Oncol, 2014;44(1):59-68. 2014 [PMID: 24220306] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol WNT5B
Uniprot