Recombinant Human TfR2 Fc Chimera Protein, CF

2 μg/lane of Recombinant Human TfR2 Fc Chimera Protein (Catalog # 10486-TF) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® Blue staining, showing bands at kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TfR2 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human TfR2 protein

  MD

  Human IgG 1 (Pro100-Lys330)

  IEGR

  Human TfR2 (Gly112-Phe801) Accession # NP_003218.2

  N-terminus

  C-terminus

• Accession #: NP_003218.2
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-117 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TfR2 Fc Chimera Protein, CF

• HFE3
• HFE3transferrin receptor protein 2
• MGC126368
• TfR2
• TFRC2
• transferrin receptor 2

Background

Transferrin Receptor 2 (TfR2 or TfR2 alpha) is an iron-binding type 2 transmembrane glycoprotein homologous to the Transferrin Receptor 1 (TfR or TfR1) (1). It forms a 215 kDa disulfide-linked homodimer and is predominantly expressed in the liver and modulates hepcidin production in response to iron (1, 2). Human TfR2 cDNA encodes 801 amino acids (aa) including an 83 aa N-terminal intracellular region, a 21 aa transmembrane domain, and a 697 aa extracellular domain (ECD). Human TfR2 ECD shares 86% and 45% aa identity with mouse TfR2 and human TfR1, respectively. An alternative splicing isoform (TfR2 beta) lacks the intracellular region, transmembrane domain and part of the extracellular regions and is expressed ubiquitously (1, 3). The Iron loaded Transferrin (holo-Transferrin) circulating in blood binds to cell-surface receptor TFR2 (4). Mutations in TFR2 can cause a form of hereditary hemochromatosis (HH, type 111), a disorder marked by chronic iron overload (5).

1. Kawabata, H. et al. (1999) J. Biol. Chem. 274:20826.
2. Trinder, D. and E. Baker (2003) Int. J. Biochem. Cell. Biol. 35:292.
3. Deaglio, S. et al. (2002) Blood 100:3782.
4. Aisen, P. et al. (1978) J. Biol Chem. 253:1930.
5. Camaschella, C. et al. (2000) Nat Genet. 25:14.

Bioinformatics

• Uniprot:
  • NP_003218.2()