Recombinant Human TFF2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TFF2 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF2 can effectively induce ERK1/2 phosphorylation.
• Source: Chinese Hamster Ovary cell line, CHO-derived human TFF2 protein
  Glu24-Tyr129
• Accession #: Q03403
• N-terminal Sequence: Glu24
• Protein/Peptide Type: Recombinant Proteins
• Gene: TFF2
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17-25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TFF2 Protein, CF

• SML1
• SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1
• SP
• Spasmolysin
• Spasmolytic Polypeptide
• TFF2
• trefoil factor 2

Background

Trefoil Factor 2 (TFF2), also known as spasmolytic peptide (SP), is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF2 is an approximately 20 kDa glycosylated peptide that plays an important role in epithelial regeneration and wound healing (1, 2). Mature human TFF2 shares 87% and 83% amino acid sequence identity with mouse and rat TFF2, respectively. TFF2 is primarily expressed by gastric mucosa of the pyloric stomach where it binds to Gastrokine 2 (3, 4). It is up-regulated in bronchiolar epithelium following exposure to allergens and is excreted into the urine at increased levels in patients with kidney stones (5, 6). TFF2 knockout mice exhibit increased gastric epithelium damage following H. pylori infection or treatment with non-steroidal anti-inflammatory drugs (7, 8). Administration of TFF2 can reduce the severity of experimental colitis (9-11). TFF2 is down-regulated in many gastric cancers, although it is up-regulated in some breast cancers (12-14). TFF2 promotes the migration of normal epithelial cells as well as tumor cells (14, 15).

1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
2. May, F.E.B. et al. (2000) Gut 46:454.
3. Madsen, J. et al. (2007) J. Histochem. Cytochem. 55:505.
4. Otto, W.R. et al. (2006) Proteomics 6:4235.
5. Nikolaidis, N.M. et al. (2006) Exp. Lung Res. 32:483.
6. Rinnert, M. et al. (2010) Cell Tissue Res. 339:639.
7. Fox, J.G. et al. (2007) Am. J. Pathol. 171:1520.
8. Farrell, J.J. et al. (2002) J. Clin. Invest. 109:193.
9. Soriano-Izquierdo, A. et al. (2004) J. Leukoc. Biol. 75:214.
10. Poulsen, S.S. et al. (2005) Regul. Pept. 126:163.
11. Tran, C.P. et al. (1999) Gut 44:636.
12. Leung, W.K. et al. (2002) J. Pathol. 197:582.
13. Kirikoshi, H. and M. Katoh (2002) Int. J. Oncol. 21:655.
14. May, F.E. et al. (2004) Peptides 25:865.
15. Oertel, M. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:418.

Publications for TFF2 (8290-TF)(1)

Publication using 8290-TF

• G Wang, X Wu, G Zhu, S Han, J Zhang Dexmedetomidine alleviates sleep-restriction-mediated exaggeration of postoperative immunosuppression via splenic TFF2 in aged mice Aging (Albany NY), 2020-03-22;12(6):5318-5335. 2020-03-22 [PMID: 32200357] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: TFF2
• Uniprot:
  • Q03403()