Recombinant Human Testican 2/SPOCK2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Testican 2/SPOCK2 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human Testican 2/SPOCK2 protein
  Glu23-Trp424, with a C-terminal 6-His tag
• Accession #: Q92563
• N-terminal Sequence: Glu23
• Protein/Peptide Type: Recombinant Proteins
• Gene: SPOCK2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Testican 2/SPOCK2 Protein, CF

• FLJ97039
• KIAA0275
• KIAA0275CWCV, and Kazal-like domains proteoglycan 2
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2
• SPOCK2
• Testican 2

Background

Testican 2 encoded by the SPOCK2 gene is a proteoglycan highly expressed in brain (1, 2). The human and mouse cDNAs predict 94% identity between the deduced amino acid sequences from the two species, indicating a conserved function (3, 4). Testican 2 contains a Ca²⁺-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. However, these domains may not be involved directly in the inhibition of targeted proteases. For example, Testican 2 is not able to inhibit MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (5). The purified rhTestican 2 is not capable of inhibiting rhMMP-14 and rhCathepsin L (R&D Systems, Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (R&D Systems, Catalog # ES001 and ES008). Nevertheless, over expression of Testican 2 is able to abrogate inhibition of MMP-14 by other testican family members and to inhibit neurite outgrowth (6, 7). 

1. Nagase, T. et al. (1996) DNA Res. 3:321.
2. Vannahme, C. et al. (1999) J. Neurochem. 73:12.
3. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
4. Okazaki, Y. et al. (2002) Nature 420:563.
5. Nakada, M. et al. (2001) Cancer Res. 61:8896.
6. Nakada, M. et al. (2003) Cancer Res. 63:3364.
7. Hartmann, U. and P. Maurer (2001) Matrix Biol. 20:23.

Publications for Testican 2/SPOCK2 (2328-PI)(1)

Publication using 2328-PI

• Wang, C;Xu, Y;Xu, M;Sun, C;Zhang, X;Tao, X;Song, T; SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury Journal of neuroinflammation 2024-02-22 [PMID: 38388415] (In vivo assay, Rat)

Bioinformatics

• Gene Symbol: SPOCK2
• Uniprot:
  • Q92563()