Recombinant Human SIRP beta 1/CD172b Protein, CF

When Recombinant Human SIRP beta 1/CD172b (Catalog # 9978-SB) isimmobilized at 1 µg/mL, Recombinant Human SP‑D (Catalog # 1920-SP) binds with anED50 of 0.1-0.8 μg/mL.

2 μg/lane of Recombinant Human SIRP beta 1/CD172b (Catalog #9978-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized by Coomassie® Blue staining, showing bands at 45-61 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SIRP beta 1/CD172b Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP beta 1/CD172b (Catalog # 9978-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED₅₀ of 0.1-0.8 μg/mL
• Source: Human embryonic kidney cell, HEK293-derived human SIRP beta 1/CD172b protein
  

  Human SIRP beta 1/CD172b (Glu30-Ala369) Accession # O00241	HH	Hemagglutinin Tag (YPYDVPDYA)
  N-terminus		C-terminus

• N-terminal Sequence: Glu30
• Structure / Form: Dimer, non covalent
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-61 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SIRP beta 1/CD172b Protein, CF

• CD172 antigen-like family member B
• CD172b antigen
• CD172b
• DKFZp686A05192
• FLJ26614
• signal-regulatory protein beta 1
• signal-regulatory protein beta-1
• SIRP beta 1
• SIRPB1
• SIRP-beta-1 isoform 3
• SIRP-BETA-1

Background

Signal-regulatory protein beta 1 (SIRP beta 1) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. The extracellular domain contains 3 intramolecular disulfide bonds and one interchain disulfide at Cys320. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). Human SIRP beta 1 shares a 57% sequence identity with mouse and rat SIRP beta 1. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5,6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma  receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

1. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
2. van den Berg, T. et al. (2008) Trends in Immunology 29:203.
3. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132
4. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
5. Dietrich J. et al. (2000) J Immunol. 164:9.
6. Brook G. et al. (2004) J Immunol. 173:2562.
7. Hayashi A. et al. (2004) J Biol Chem. 279:29450.
8. Fournier B. et al. (2012) J. Biol. Chem. 287:19386.
9. Seiffert M. et al. (2001) Blood 97:2741.

Publications for SIRP beta 1/CD172b (9978-SB)(1)

Publication using 9978-SB

• YV Vladimirov, MK Mølmer, KW Antonsen, N Møller, N Rittig, MC Nielsen, HJ Møller A New Serum Macrophage Checkpoint Biomarker for Innate Immunotherapy: Soluble Signal-Regulatory Protein Alpha (sSIRPalpha) Biomolecules, 2022-07-04;12(7):. 2022-07-04 [PMID: 35883493] (ELISA Standard, Human)