Recombinant Human sFRP-3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human sFRP-3 Protein, CF Summary

Additional Information
A New and Improved rh sFRP-3 is Available! It has a lower endotoxin specification, ~50 fold better activity, and no tag!
Details of Functionality
Measured by its ability to inhibit proliferation in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1‑4 µg/mL.
Optimal concentrations should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human sFRP-3 protein
Ala33-Asn325, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ala33
Protein/Peptide Type
Recombinant Proteins
Gene
FRZB
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38 kDa, reducing conditions
Publications
Read Publications using
192-SF/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human sFRP-3 Protein, CF

  • FIZ
  • FREOS1
  • Frezzled
  • Fritz
  • Frizzled-related protein 1
  • frizzled-related protein
  • FRP
  • FRP-3
  • FRZB
  • Frzb1
  • FRZB-1
  • FRZB1sFRP-3
  • FRZB-PEN
  • FZRB
  • hFIZ
  • secreted frizzled-related protein 3
  • sFRP3
  • sFRP-3
  • SFRP3frezzled
  • SRFP3frizzled homolog-related

Background

Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas.

The N-terminal portion of the protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.

  1. Hoang, et al. (1996) J. Biol. Chem. 271:26131.
  2. Leyns, et al. (1997) Cell 88:747.
  3. Wang, et al. (1997) Cell 88:757.
  4. Mayr, et al. (1997) Mech. Dev. 63:109.
  5. Rattner, et al. (1997) PNAS 94:2859.

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Publications for sFRP-3/FRZB (192-SF/CF)(4)

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Bioinformatics

Gene Symbol FRZB
Uniprot