Recombinant Human SCARA5 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human SCARA5 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834. The ED₅₀ for this effect is 0.4-2 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived human SCARA5 protein
  Arg83-His495, with an N-terminal 6-His tag
• Accession #: Q6ZMJ2
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: SCARA5
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 45.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 66-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCARA5 Protein, CF

• FLJ23907
• MGC45780
• SCARA5
• scavenger receptor class A, member 5 (putative)
• Tesr
• testis expressed scavenger receptor
• UNQ2938/PRO28700

Background

Scavenger receptor A5 (SCARA5) is also known as testis expressed scavenger receptor (Tesr). Class A scavenger receptors, including CL-P1, MARCO, SCARA3, and SR-A1, are type II transmembrane proteins that contain a collagenous stalk (1 - 3). Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) domain (4 - 6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. It shares 23% - 34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).

1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
2. Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
3. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
4. Sarraj, M.A. et al. (2005) Dev. Dyn. 234:1026.
5. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
6. Accession # Q6ZMJ2.

Bioinformatics

• Gene Symbol: SCARA5
• Uniprot:
  • Q6ZMJ2()